Effects of Substrate-Coating Materials on the Wound-Healing Process

The wound-healing assay is commonly and widely used for investigating collective cell migration under various physical and chemical stimuli. Substrate-coating materials are shown to affect the wound-healing process in a cell-type dependent manner. However, experiment-to-experiment variations make it...

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Bibliographic Details
Published in:Materials 2019-08, Vol.12 (17), p.2775
Main Authors: Lin, Jin-Young, Lo, Kai-Yin, Sun, Yung-Shin
Format: Article
Language:English
Subjects:
Assaying
Cell adhesion & migration
Cell culture
Coating
Experiments
Fibroblasts
Gelatin
Lysine
Protective coatings
Substrates
Wound healing
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Summary:The wound-healing assay is commonly and widely used for investigating collective cell migration under various physical and chemical stimuli. Substrate-coating materials are shown to affect the wound-healing process in a cell-type dependent manner. However, experiment-to-experiment variations make it difficult to compare results from different assays. In this paper, a modified barrier wound-healing assay was reported for studying the wound-healing process on different substrates in one single petri dish. In short, half of a dish was covered with the tape, and coating materials, poly-l-lysine and gelatin, were applied to the surface. After peeling off the tape, half of the surface was coated with the desired material. Then a customized barrier was placed inside the dish to create the wound. The results indicated that surface coating did not affect cell proliferation/viability, and the wound-healing rate increased in coated surfaces compared to uncoated ones. The present study provides a platform for further understanding the mechanisms of substrate coating-dependent wound-healing processes.
ISSN:1996-1944
1996-1944
DOI:10.3390/ma12172775