Phenotypic characterisation of regulatory T cells in dogs reveals signature transcripts conserved in humans and mice

Regulatory T cells (Tregs) are a double-edged regulator of the immune system. Aberrations of Tregs correlate with pathogenesis of inflammatory, autoimmune and neoplastic disorders. Phenotypically and functionally distinct subsets of Tregs have been identified in humans and mice on the basis of their...

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Bibliographic Details
Published in:Scientific reports 2019-09, Vol.9 (1), p.13478-12, Article 13478
Main Authors: Wu, Ying, Chang, Yu-Mei, Stell, Anneliese J., Priestnall, Simon L., Sharma, Eshita, Goulart, Michelle R., Gribben, John, Xia, Dong, Garden, Oliver A.
Format: Article
Language:English
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Animals
Antigens
Antigens, Surface
Biomarkers
CD25 antigen
CD4 antigen
Conserved Sequence
Dogs
Evolution, Molecular
Gene Expression Profiling
Humanities and Social Sciences
Humans
Immune system
Immunophenotyping
Immunoregulation
Lymphocytes
Lymphocytes T
Mice
Monoclonal antibodies
multidisciplinary
Phenotypes
Science
Science (multidisciplinary)
Signal Transduction
Surface antigens
T-Lymphocyte Subsets - immunology
T-Lymphocyte Subsets - metabolism
T-Lymphocytes, Regulatory - immunology
T-Lymphocytes, Regulatory - metabolism
Transcription
Transcriptome
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Summary:Regulatory T cells (Tregs) are a double-edged regulator of the immune system. Aberrations of Tregs correlate with pathogenesis of inflammatory, autoimmune and neoplastic disorders. Phenotypically and functionally distinct subsets of Tregs have been identified in humans and mice on the basis of their extensive portfolios of monoclonal antibodies (mAb) against Treg surface antigens. As an important veterinary species, dogs are increasingly recognised as an excellent model for many human diseases. However, insightful study of canine Tregs has been restrained by the limited availability of mAb. We therefore set out to characterise CD4 + CD25 high T cells isolated ex vivo from healthy dogs and showed that they possess a regulatory phenotype, function, and transcriptomic signature that resembles those of human and murine Tregs. By launching a cross-species comparison, we unveiled a conserved transcriptomic signature of Tregs and identified that transcript hip1 may have implications in Treg function.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-019-50065-8