Detection of Human Anti-Trypanosoma cruzi Antibody with Recombinant Fragmented Ribosomal P Protein

Chagas disease is caused by the protozoan parasite Trypanosoma cruzi, and is endemic in many Latin American countries. Diagnosis is based on serologic testing and the WHO recommends two or more serological tests for confirmation. Acidic ribosomal P protein of T. cruzi showed strong reactivity agains...

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Published in:Korean journal of parasitology 2019, Hosts and Diseases, 57(4), , pp.435-437
Main Authors: Kim, Yeong Hoon, Yang, Zhaoshou, Lee, Jihoo, Ahn, Hye-Jin, Chong, Chom-Kyu, Maricondi, Wagner, Dias, Ronaldo F, Nam, Ho-Woo
Format: Article
Language:English
Subjects:
Antibodies, Protozoan - blood
Blotting, Western
Brazil
Brief Communication
Chagas Disease - blood
Chagas Disease - diagnosis
Chagas Disease - immunology
Endemic Diseases
Humans
Recombinant Proteins - immunology
Ribosomal Proteins - immunology
Trypanosoma cruzi - immunology
예방의학
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Summary:Chagas disease is caused by the protozoan parasite Trypanosoma cruzi, and is endemic in many Latin American countries. Diagnosis is based on serologic testing and the WHO recommends two or more serological tests for confirmation. Acidic ribosomal P protein of T. cruzi showed strong reactivity against positive sera of patients, and we cloned the protein after fragmenting it to enhance its antigenicity and solubility. Twelve positive sera of Chagas disease patients were reacted with the fragmented ribosomal P protein using western blot. Detection rate and density for each fragment were determined. Fragments F1R1, F1R2, and F2R1 showed 100% rate of detection, and average density scoring of 2.00, 1.67, and 2.42 from a maximum of 3.0, respectively. Therefore, the F2R1 fragment of the ribosomal P protein of T. cruzi could be a promising antigen to use in the diagnosis of Chagas disease in endemic regions with high specificity and sensitivity.
ISSN:0023-4001
2982-5164
1738-0006
2982-6799
DOI:10.3347/kjp.2019.57.4.435