Optical detection of harmonic oscillations in fluorescent dye-loaded microbubbles ensonified by ultrasound

A new optical contrast agent has been developed by exposing dye-loaded microbubbles to a rapidly-cooled thermal treatment to homogenize the dye distribution across the surface. Ultrasound causes these microbubbles to oscillate in size which changes the self-quenching efficiency of the dye molecules...

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Bibliographic Details
Published in:Optics letters 2015-06, Vol.40 (12), p.2834-2837
Main Authors: Schutt, Carolyn E, Ibsen, Stuart, Benchimol, Michael, Hsu, Mark, Esener, Sadik
Format: Article
Language:English
Subjects:
Contrast Media
Dyes
Efficiency
Fluorescence
Fluorescent Dyes
Harmonics
Hot Temperature
Microbubbles
Modulation
Resonant frequency
Signal to noise ratio
Ultrasonic Waves
Ultrasound
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Summary:A new optical contrast agent has been developed by exposing dye-loaded microbubbles to a rapidly-cooled thermal treatment to homogenize the dye distribution across the surface. Ultrasound causes these microbubbles to oscillate in size which changes the self-quenching efficiency of the dye molecules creating a "blinking" signal. We demonstrate for the first time that these microbubbles can reproducibly generate second, third, and even fourth harmonic fluorescence intensity modulations, in addition to the fundamental frequency of the driving ultrasound. Detecting these harmonic signals could produce a higher signal-to-noise ratio for fluorescence imaging in medical applications by allowing fundamental frequency interference and artifacts to be filtered out.
ISSN:0146-9592
1539-4794
DOI:10.1364/OL.40.002834