Pyrethroid exposure alters internal and cuticle surface bacterial communities in Anopheles albimanus

• Published in: The ISME Journal 2019-10, Vol.13 (10), p.2447-2464
• Main Authors: Dada, Nsa, Lol, Juan C., Benedict, Ana Cristina, López, Francisco, Sheth, Mili, Dzuris, Nicole, Padilla, Norma, Lenhart, Audrey
• Format: Article
• Language: English
• Subjects: 45/22; 45/41; 45/47; 45/77; 631/158/855; 631/208/212/2142; 631/326/2565/2134; 631/337; 631/601/1737; Adults; Animals; Anopheles - drug effects; Anopheles - microbiology; Anopheles albimanus; Aquatic insects; Bacteria; Bacteria - classification; Bacteria - drug effects; Bacteria - genetics; Bacteria - isolation & purification; Biomedical and Life Sciences; Communities; Composition; Culicidae; Cuticles; Deltamethrin; Ecology; Evolutionary Biology; Exposure; Female; Insecticide resistance; Insecticides; Insecticides - pharmacology; Larva - drug effects; Larva - microbiology; Life Sciences; Malaria; Male; Microbial Ecology; Microbial Genetics and Genomics; Microbiology; Microbiota; Microbiota - drug effects; Microorganisms; Mosquito Vectors - drug effects; Mosquito Vectors - microbiology; Mosquitoes; Niches; Nitriles - pharmacology; Permethrin; Permethrin - pharmacology; Pesticide resistance; Progeny; Pseudomonas fragi; Pyrethrins - pharmacology; Pyrethroids; Vector-borne diseases
• ISSN: 1751-7362; 1751-7370
• DOI: 10.1038/s41396-019-0445-5

A deeper understanding of the mechanisms underlying insecticide resistance is needed to mitigate its threat to malaria vector control. Following previously identified associations between mosquito microbiota and insecticide resistance, we demonstrate for the first time, the effects of pyrethroid exposure on the microbiota of F 1 progeny of field-collected Anopheles albimanus . Larval and adult mosquitoes were exposed to the pyrethroids alphacypermethrin (only adults), permethrin, and deltamethrin. While there were no significant differences in bacterial composition between insecticide-resistant and insecticide-susceptible mosquitoes, bacterial composition between insecticide-exposed and non-exposed mosquitoes was significantly different for alphacypermethrin and permethrin exposure. Along with other bacterial taxa not identified to species, Pantoea agglomerans (a known insecticide-degrading bacterial species) and Pseudomonas fragi were more abundant in insecticide-exposed compared to non-exposed adults, demonstrating that insecticide exposure can alter mosquito bacterial communities. We also show for the first time that the cuticle surfaces of both larval and adult An. albimanus harbor more diverse bacterial communities than their internal microbial niches. Together, these findings demonstrate how insecticide pressure could be selecting for certain bacteria within mosquitoes, especially insecticide-metabolizing bacteria, thus potentially contributing to insecticide resistance.