Phosphorylation-Mediated Dynamics of Nitrate Transceptor NRT1.1 Regulate Auxin Flux and Nitrate Signaling in Lateral Root Growth

• Published in: Plant physiology (Bethesda) 2019-10, Vol.181 (2), p.480-498
• Main Authors: Zhang, Xi, Cui, Yaning, Yu, Meng, Su, Bodan, Gong, Wei, Baluška, František, Komis, George, Šamaj, Jozef, Shan, Xiaoyi, Lin, Jinxing
• Format: Article
• Language: English
• Subjects: CELL BIOLOGY
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.19.00346

The dual-affinity nitrate transceptor NITRATE TRANSPORTER1.1 (NRT1.1) has two modes of transport and signaling, governed by Thr-101 (T101) phosphorylation. NRT1.1 regulates lateral root (LR) development by modulating nitratedependent basipetal auxin export and nitrate-mediated signal transduction. Here, using the Arabidopsis (Arabidopsis thaliana) NRT1.1T101D phosphomimetic and NRT1.1T101A nonphosphorylatable mutants, we found that the phosphorylation state of NRT1.1 plays a key role in NRT1.1 function during LR development. Single-particle tracking revealed that phosphorylation affected NRT1.1 spatiotemporal dynamics. The phosphomimetic NRT1.1T101D form showed fast lateral mobility and membrane partitioning that facilitated auxin flux under low-nitrate conditions. By contrast, nonphosphorylatable NRT1.1T101A showed low lateral mobility and oligomerized at the plasma membrane (PM), where it induced endocytosis via the clathrin-mediated endocytosis and microdomain-mediated endocytosis pathways under high-nitrate conditions. These behaviors promoted LR development by suppressing NRT1.1-controlled auxin transport on the PM and stimulating Ca²⁺-ARABIDOPSIS NITRATE REGULATED1 signaling from the endosome.