Multiparametric radiobiological assays show that variation of X-ray energy strongly impacts relative biological effectiveness: comparison between 220 kV and 4 MV

Based on classic clonogenic assay, it is accepted by the scientific community that, whatever the energy, the relative biological effectiveness of X-rays is equal to 1. However, although X-ray beams are widely used in diagnosis, interventional medicine and radiotherapy, comparisons of their energies...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2019-10, Vol.9 (1), p.14328-10, Article 14328
Main Authors: Paget, Vincent, Ben Kacem, Mariam, Dos Santos, Morgane, Benadjaoud, Mohamed A., Soysouvanh, Frédéric, Buard, Valérie, Georges, Tarlet, Vaurijoux, Aurélie, Gruel, Gaëtan, François, Agnès, Guipaud, Olivier, Milliat, Fabien
Format: Article
Language:English
Subjects:
13
13/31
14/34
38/77
45/77
631/80/509
631/80/641
631/80/82
Cell proliferation
Cell Survival - radiation effects
Colony-Forming Units Assay
DNA Breaks, Double-Stranded - radiation effects
Endothelial cells
Energy
Flow cytometry
Genetic Markers - radiation effects
Histones - genetics
Histones - radiation effects
Human Umbilical Vein Endothelial Cells
Humanities and Social Sciences
Humans
Life Sciences
Linear Energy Transfer
multidisciplinary
Photons
Photons - adverse effects
Proof of Concept Study
Radiation therapy
Relative Biological Effectiveness
Science
Science (multidisciplinary)
Senescence
Umbilical vein
X-rays
X-Rays - adverse effects
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Based on classic clonogenic assay, it is accepted by the scientific community that, whatever the energy, the relative biological effectiveness of X-rays is equal to 1. However, although X-ray beams are widely used in diagnosis, interventional medicine and radiotherapy, comparisons of their energies are scarce. We therefore assessed in vitro the effects of low- and high-energy X-rays using Human umbilical vein endothelial cells (HUVECs) by performing clonogenic assay, measuring viability/mortality, counting γ-H2AX foci, studying cell proliferation and cellular senescence by flow cytometry and by performing gene analysis on custom arrays. Taken together, excepted for γ-H2AX foci counts, these experiments systematically show more adverse effects of high energy X-rays, while the relative biological effectiveness of photons is around 1, whatever the quality of the X-ray beam. These results strongly suggest that multiparametric analysis should be considered in support of clonogenic assay.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-019-50908-4