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Distinct Pathogenic Patterns of Burkholderia pseudomallei Isolates Selected from Caenorhabditis elegans and Dictyostelium discoideum Models
is a selective agent that causes septic melioidosis and exhibits a broad range of lethal doses in animals. Host cellular virulence and phagocytic resistance are pathologic keys of . We first proposed as the host cellular virulence model to mimic bacterial virulence against mammals and second establi...
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Published in: | The American journal of tropical medicine and hygiene 2019-01, Vol.101 (4), p.736-745 |
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Main Authors: | , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that cite this one |
Online Access: | Get full text |
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Summary: | is a selective agent that causes septic melioidosis and exhibits a broad range of lethal doses in animals. Host cellular virulence and phagocytic resistance are pathologic keys of
. We first proposed
as the host cellular virulence model to mimic bacterial virulence against mammals and second established the resistance of
to predation by
as the phagocytosis model. The saprophytic sepsis-causing
sp. (
,
,
, and
) exhibited different virulence patterns in both simple models, but
was the most toxic. Using both models, attenuated isolates of
were selected from a transposon-mutant library and a panel of environmental isolates and reconfirmed by in vitro mouse peritoneal exudate cell association and invasion assays. The distinct pathological patterns of melioidosis were inducted by different selected
isolates. Fatal melioidosis was induced by the isolates with high virulence in both simple models within 4-5 day, whereas the low-virulence isolates resulted in prolonged survival greater than 30 day. Infection with the isolates having high resistance to
predation but a low
killing effect led to 83% of mice with neurologic melioidosis. By contrast, infection with the isolates having low resistance to
predation but high
killing effect led to 20% cases with inflammation in the salivary glands. Our results indicated that individual
isolates selected from simple biological models contribute differently to disease progression and/or tissue tropism. |
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ISSN: | 0002-9637 1476-1645 |
DOI: | 10.4269/ajtmh.19-0052 |