Blue laser light inhibits biofilm formation in vitro and in vivo by inducing oxidative stress

Resolution of bacterial infections is often hampered by both resistance to conventional antibiotic therapy and hiding of bacterial cells inside biofilms, warranting the development of innovative therapeutic strategies. Here, we report the efficacy of blue laser light in eradicating Pseudomonas aerug...

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Bibliographic Details
Published in:NPJ biofilms and microbiomes 2019-10, Vol.5 (1), p.29-11, Article 29
Main Authors: Rupel, Katia, Zupin, Luisa, Ottaviani, Giulia, Bertani, Iris, Martinelli, Valentina, Porrelli, Davide, Vodret, Simone, Vuerich, Roman, Passos da Silva, Daniel, Bussani, Rossana, Crovella, Sergio, Parsek, Matthew, Venturi, Vittorio, Di Lenarda, Roberto, Biasotto, Matteo, Zacchigna, Serena
Format: Article
Language:English
Subjects:
631/326/46
692/700/3032
Animals
Bacteria
Biofilms
Biofilms - growth & development
Biofilms - radiation effects
Biomedical and Life Sciences
Cell Line
Culture Media
Disease Models, Animal
Humans
Lasers
Life Sciences
Light
Mammalian cells
Medical innovations
Medical Microbiology
Mice, Inbred C57BL
Microbial Ecology
Microbial Genetics and Genomics
Microbiology
Models, Biological
Oxidative Stress
Phototherapy
Pseudomonas aeruginosa - growth & development
Pseudomonas aeruginosa - radiation effects
Pseudomonas Infections - therapy
Radiotherapy - methods
Skin
Toxicity
Treatment Outcome
Wound infection
Wound Infection - therapy
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Summary:Resolution of bacterial infections is often hampered by both resistance to conventional antibiotic therapy and hiding of bacterial cells inside biofilms, warranting the development of innovative therapeutic strategies. Here, we report the efficacy of blue laser light in eradicating Pseudomonas aeruginosa cells, grown in planktonic state, agar plates and mature biofilms, both in vitro and in vivo, with minimal toxicity to mammalian cells and tissues. Results obtained using knock-out mutants point to oxidative stress as a relevant mechanism by which blue laser light exerts its anti-microbial effect. Finally, the therapeutic potential is confirmed in a mouse model of skin wound infection. Collectively, these data set blue laser phototherapy as an innovative approach to inhibit bacterial growth and biofilm formation, and thus as a realistic treatment option for superinfected wounds.
ISSN:2055-5008
2055-5008
DOI:10.1038/s41522-019-0102-9