SPIONs/3D SiSBA-16 based Multifunctional Nanoformulation for target specific cisplatin release in colon and cervical cancer cell lines

• Published in: Scientific reports 2019-10, Vol.9 (1), p.14523-12, Article 14523
• Main Authors: Jermy, B. Rabindran, Alomari, Munther, Ravinayagam, Vijaya, Almofty, Sarah Ameen, Akhtar, Sultan, Borgio, J. Francis, AbdulAzeez, Sayed
• Format: Article
• Language: English
• Subjects: 13/2; 631/154/140; 692/699/67/1059; Acrylic acid; Adsorption; Antineoplastic Agents - administration & dosage; Apoptosis; Cell Line, Tumor; Cervical cancer; Cervix; Chemotherapy; Cisplatin; Cisplatin - administration & dosage; Colon cancer; Colonic Neoplasms - drug therapy; Colorectal cancer; Cytotoxicity; Dialysis; Drug Carriers; Drug delivery; Female; HCT116 Cells; HEK293 Cells; HeLa Cells; Humanities and Social Sciences; Humans; Iron oxides; Kidney - drug effects; Magnetic Resonance Imaging; Magnetite Nanoparticles - chemistry; Microscopy, Electron, Transmission; multidisciplinary; Nanotechnology; Science; Science (multidisciplinary); Silica; Silicon Dioxide - chemistry; Tumor cell lines; Uterine Cervical Neoplasms - drug therapy; X-Ray Diffraction
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-019-51051-w

Multifunctional nanomaterials can be used for dual applications: drug delivery as well as in bioimaging. In current study, we investigated potential use of silica based supports; 3D cage type SiSBA-16 (S-16), monodispersed hydrophilic spherical silica (HYPS) and mesocellular foam (MSU-F) for cisplatin (Cp) delivery. To obtain magnetic resonance characteristics, 10 wt% iron oxide was loaded through enforced adsorption technique. For pH stimuli responsive release of Cp, 10 wt%SPIONs/S-16 was functionalized with 3-(Aminopropyl)triethoxysilane (A) and poly acrylic acid (PAA) termed as 10 wt%SPIONs/S-16-A-Cp and 10 wt%SPIONs/S-16-APAA-Cp. By TEM analysis, the average diameter of the SPIONs was found to range between 10–60 nm. VSM analysis showed saturation magnetization over S-16, HYPS and MSU-F were in the following order: 10 wt%SPIONs/HYPS (4.08 emug −1 ) > 10 wt%SPIONs /S-16 (2.39 emug −1 ) > 10 wt%SPIONs/MSU-F (0.23 emug −1 ). Cp release study using dialysis membrane in PBS solution over 10 wt%SPIONs/S-16 nanoformulations showed highest cumulative release (65%) than 10 wt%SPIONs/MSU-F-A-Cp (63%), 10 wt%SPIONs/HYPS-A-Cp (58%), and Cp-F127/S-16 (53%), respectively. 10 wt%SPIONs/S-16-A-Cp and 10 wt%SPIONs/S-16-APAA-Cp were evaluated for in vitro target anticancer efficiency in human cancer cell lines (colon cancer (HCT 116), cervical cancer (HeLa)) and normal cells (Human embryonic kidney cells (HEK293) using MTT and DAPI staining. 10 wt%SPIONs/S-16-A-Cp treated Hela and HCT116 cancerous cell lines showed significant control of cell growth, apoptotic activity and less cytotoxic effect as compared to Cp and 10 wt%SPIONs/S-16. Target specific Cp release in the cells shows that 10 wt%SPIONs/S-16-A-Cp can be easily upgraded for magnetic resonance imaging capability.