The interplay between chondrocyte spheroids and mesenchymal stem cells boosts cartilage regeneration within a 3D natural-based hydrogel

Articular cartilage (AC) lacks the ability to self-repair and cell-based approaches, primarily based on using chondrocytes and mesenchymal stem cells (MSCs), are emerging as effective technology to restore cartilage functionality, because cells synergic functionality may support the maintenance of c...

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Bibliographic Details
Published in:Scientific reports 2019-10, Vol.9 (1), p.14630-12, Article 14630
Main Authors: Scalzone, Annachiara, Ferreira, Ana M., Tonda-Turo, Chiara, Ciardelli, Gianluca, Dalgarno, Kenny, Gentile, Piergiorgio
Format: Article
Language:English
Subjects:
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Cartilage (articular)
Cartilage, Articular - cytology
Cartilage, Articular - physiology
Cartilage, Articular - ultrastructure
CD44 antigen
Cell Communication - physiology
Cell culture
Cell Culture Techniques - methods
Cell Line
Cells, Cultured
Chitosan
Chitosan - chemistry
Chondrocytes
Chondrocytes - physiology
Coculture Techniques - methods
Collagen
Extracellular matrix
Glycosaminoglycans
Histology
Humanities and Social Sciences
Humans
Hydrogels
Hydrogels - chemistry
Immunofluorescence
Mesenchymal stem cells
Mesenchymal Stem Cells - physiology
Mesenchyme
Microscopy, Electron, Scanning
multidisciplinary
Phenotypes
Porosity
Regeneration - physiology
Science
Science (multidisciplinary)
Spheroids
Spheroids, Cellular
Stem cells
Tissue Engineering - methods
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Summary:Articular cartilage (AC) lacks the ability to self-repair and cell-based approaches, primarily based on using chondrocytes and mesenchymal stem cells (MSCs), are emerging as effective technology to restore cartilage functionality, because cells synergic functionality may support the maintenance of chondrogenic phenotype and promote extracellular matrix regeneration. This work aims to develop a more physiologically representative co-culture system to investigate the influence of MSCs on the activity of chondrocytes. A thermo-sensitive chitosan-based hydrogel, ionically crosslinked with β–glycerophosphate, is optimised to obtain sol/gel transition at physiological conditions within 5 minutes, high porosity with pores diameter
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-019-51070-7