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Single-tube, dual channel pentaplexing for the identification of Candida strains associated with human infection

Invasive candidiasis is one of the most common nosocomial fungal infections worldwide. Delayed implementation of effective antifungal treatment caused by inefficient Candida diagnosis contributes to its notoriously high mortality rates. The availability of better Candida diagnostic tools would posit...

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Published in:Scientific reports 2019-10, Vol.9 (1), p.14692-14, Article 14692
Main Authors: Jainlabdin, Mohd Hanif, Batra, Ambalika, Sánchez Paredes, Edith, Hernández Hernández, Francisca, Fu, Guoliang, Tovar-Torres, Jorge
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description Invasive candidiasis is one of the most common nosocomial fungal infections worldwide. Delayed implementation of effective antifungal treatment caused by inefficient Candida diagnosis contributes to its notoriously high mortality rates. The availability of better Candida diagnostic tools would positively impact patient outcomes. Here, we report on the development of a single-tube, dual channel pentaplex molecular diagnostic assay based on Multiplex Probe Amplification (MPA) technology. It allows simultaneous identification of C . auris , C . glabrata and C . krusei , at species-level as well as of six additional albicans and non- albicans pathogenic Candida at genus level. The assay overcomes the one-channel one-biomarker limitation of qPCR-based assays. Assay specificities are conferred by unique biomarker probe pairs with characteristic melting temperatures; post-amplification melting curve analysis allows simple identification of the infectious agent. Alerting for the presence of C . auris , the well-characterised multi-drug resistant outbreak strain, will facilitate informed therapy decisions and aid antifungal stewardship. The MPA -Candida assay can also be coupled to a pan-Fungal assay when differentiation between fungal and bacterial infections might be desirable. Its multiplexing capacity, detection range, specificity and sensitivity suggest the potential use of this novel MPA- Candida assay in clinical diagnosis and in the control and management of hospital outbreaks.
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subjects 38/22
38/71
38/77
631/326/193
631/337
692/308/2778
9/10
Antifungal Agents - therapeutic use
Antimicrobial Stewardship
Bacterial diseases
Bacterial infections
Biomarkers
Biomarkers - analysis
Candida
Candida - classification
Candida - genetics
Candidiasis
Candidiasis, Invasive - diagnosis
Candidiasis, Invasive - drug therapy
Candidiasis, Invasive - microbiology
Diagnosis
DNA, Fungal - genetics
Drug resistance
Drug Resistance, Multiple, Fungal
Fungal infections
Hospitals
Humanities and Social Sciences
Humans
Limit of Detection
Melting
Melting curve
Molecular Diagnostic Techniques - methods
multidisciplinary
Multidrug resistance
Multiplex Polymerase Chain Reaction - methods
Nosocomial infection
Outbreaks
Real-Time Polymerase Chain Reaction - methods
Science
Science (multidisciplinary)
Sensitivity and Specificity
Transition Temperature
title Single-tube, dual channel pentaplexing for the identification of Candida strains associated with human infection
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