Nitric Oxide-Dependent Production of cGMP Supports the Survival of Rat Embryonic Motor Neurons Cultured with Brain-Derived Neurotrophic Factor

Trophic factor deprivation induces neuronal nitric oxide synthase (NOS) and apoptosis of rat embryonic motor neurons in culture. We report here that motor neurons constitutively express endothelial NOS that helps support the survival of motor neurons cultured with brain-derived neurotrophic factor (...

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Bibliographic Details
Published in:The Journal of neuroscience 1998-05, Vol.18 (10), p.3708-3714
Main Authors: Estevez, Alvaro G, Spear, Nathan, Thompson, J. Anthony, Cornwell, Trudy L, Radi, Rafael, Barbeito, Luis, Beckman, Joseph S
Format: Article
Language:English
Subjects:
8-Bromo Cyclic Adenosine Monophosphate - pharmacology
Amino Acid Chloromethyl Ketones - pharmacology
Animals
Apoptosis - drug effects
Apoptosis - physiology
Brain-Derived Neurotrophic Factor - pharmacology
Cell Survival - drug effects
Cell Survival - physiology
Cells, Cultured
Cyclic GMP - metabolism
Cysteine Proteinase Inhibitors - pharmacology
Enzyme Inhibitors - pharmacology
Fetus - cytology
Guanylate Cyclase - antagonists & inhibitors
Guanylate Cyclase - metabolism
Motor Neurons - cytology
Motor Neurons - drug effects
Motor Neurons - enzymology
NG-Nitroarginine Methyl Ester - pharmacology
Nitric Oxide - metabolism
Nitric Oxide Synthase - metabolism
Oligopeptides - pharmacology
Oxadiazoles - pharmacology
Quinoxalines - pharmacology
Rats
Solubility
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Summary:Trophic factor deprivation induces neuronal nitric oxide synthase (NOS) and apoptosis of rat embryonic motor neurons in culture. We report here that motor neurons constitutively express endothelial NOS that helps support the survival of motor neurons cultured with brain-derived neurotrophic factor (BDNF) by activating the nitric oxide-dependent soluble guanylate cyclase. Exposure of BDNF-treated motor neurons to nitro-L-arginine methyl ester (L-NAME) decreased cell survival 40-50% 24 hr after plating. Both low steady-state concentrations of exogenous nitric oxide (
ISSN:0270-6474
1529-2401
DOI:10.1523/jneurosci.18-10-03708.1998