Dynamic Regulation of RGS2 Suggests a Novel Mechanism in G-Protein Signaling and Neuronal Plasticity

• Published in: The Journal of neuroscience 1998-09, Vol.18 (18), p.7178-7188
• Main Authors: Ingi, Tatsuya, Krumins, Andrejs M, Chidiac, Peter, Brothers, Greg M, Chung, Stephen, Snow, Bryan E, Barnes, Carol A, Lanahan, Anthony A, Siderovski, David P, Ross, Elliott M, Gilman, Alfred G, Worley, Paul F
• Format: Article
• Language: English
• Subjects: Animals; Calcium-Calmodulin-Dependent Protein Kinases - metabolism; Cerebral Cortex - chemistry; Cerebral Cortex - cytology; Cerebral Cortex - enzymology; Cocaine - pharmacology; COS Cells - chemistry; COS Cells - enzymology; Dopamine Antagonists - pharmacology; Dopamine Uptake Inhibitors - pharmacology; Female; Gene Expression - drug effects; Gene Expression - physiology; Genes, Immediate-Early - physiology; GTP Phosphohydrolases - metabolism; GTP-Binding Proteins - physiology; Haloperidol - pharmacology; Hippocampus - chemistry; Hippocampus - cytology; Hippocampus - enzymology; Hydrolysis; Lipid Metabolism; Male; Neuronal Plasticity - physiology; Neurons - chemistry; Neurons - drug effects; Neurons - enzymology; Rats; Rats, Inbred F344; Rats, Sprague-Dawley; Receptors, Muscarinic - physiology; RNA, Messenger - metabolism; Signal Transduction - drug effects; Signal Transduction - physiology; Synaptic Transmission - drug effects; Synaptic Transmission - physiology
• ISSN: 0270-6474; 1529-2401
• DOI: 10.1523/jneurosci.18-18-07178.1998

Long-term neuronal plasticity is known to be dependent on rapid de novo synthesis of mRNA and protein, and recent studies provide insight into the molecules involved in this response. Here, we demonstrate that mRNA encoding a member of the regulator of G-protein signaling (RGS) family, RGS2, is rapidly induced in neurons of the hippocampus, cortex, and striatum in response to stimuli that evoke plasticity. Although several members of the RGS family are expressed in brain with discrete neuronal localizations, RGS2 appears unique in that its expression is dynamically responsive to neuronal activity. In biochemical assays, RGS2 stimulates the GTPase activity of the alpha subunit of Gq and Gi1. The effect on Gi1 was observed only after reconstitution of the protein in phospholipid vesicles containing M2 muscarinic acetylcholine receptors. RGS2 also inhibits both Gq- and Gi-dependent responses in transfected cells. These studies suggest a novel mechanism linking neuronal activity and signal transduction.