Evaluating NISCH and CDH1 Promoter Hypermethylation in Nonsmokers, Cancer Free Smokers and Lung Cancer Patients: A Case Control Study

Lung cancer has very high mortality due to late stage diagnosis not amenable to curative resection. Cancer specific methylation patterns of tumor suppressor genes may precede precursor lesions of lung cancer. Our aim was to evaluate the promoter hypermethylation of tumor suppressor gene NISCH and CD...

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Bibliographic Details
Published in:Indian journal of clinical biochemistry 2019-10, Vol.34 (4), p.458-464
Main Authors: Krishnamurthy, Kritika, Mishra, T. K., Saxena, Alpana, Daga, M. K., Khurana, Nita, Masroor, Mirza, Jamatia, Elvia
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Bisulfite
Cancer
Cancer patients
Chemistry/Food Science
Development and progression
Diagnosis
DNA methylation
E-cadherin
Enzyme-linked immunosorbent assay
Ethylenediaminetetraacetic acid
Health aspects
Health risk assessment
Life Sciences
Lung cancer
Methylation
Microbiology
Mortality
Oncology, Experimental
Original
Original Research Article
Pathology
Patients
Smokers
Smoking
Sulfites
Tumor suppressor genes
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Summary:Lung cancer has very high mortality due to late stage diagnosis not amenable to curative resection. Cancer specific methylation patterns of tumor suppressor genes may precede precursor lesions of lung cancer. Our aim was to evaluate the promoter hypermethylation of tumor suppressor gene NISCH and CDH1 in cfDNA from plasma of lung cancer patients and its possible correlation with smoking status and various clinicopathological parameters. Forty histopathologically confirmed lung cancer cases, thirty smoker and thirty nonsmoker controls were enrolled. Plasma cfDNA was extracted and subjected to bisulfite treatment followed by MS-PCR. Serum nischarin levels were estimated by ELISA. The frequency of promoter hypermethylation of NISCH and CDH1 was significantly higher in lung cancer patients as compared to lifelong non-smoker controls ( p  
ISSN:0970-1915
0974-0422
DOI:10.1007/s12291-018-0767-5