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Organic solvent-free extraction of carotenoids from carrot bio-waste and its physico-chemical properties

The bio-wastes (like peels, seeds, etc.) from food industry are rich source of bio-active components, but are poorly managed. In present study, carotenoids were extracted from carrot pomace using ultrasonication and high shear dispersion techniques and flaxseed oil as green solvent (green biorefiner...

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Published in:Journal of food science and technology 2019-10, Vol.56 (10), p.4678-4687
Main Authors: Tiwari, Swati, Upadhyay, Neelam, Singh, Ashish Kumar, Meena, Ganga Sahay, Arora, Sumit
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description The bio-wastes (like peels, seeds, etc.) from food industry are rich source of bio-active components, but are poorly managed. In present study, carotenoids were extracted from carrot pomace using ultrasonication and high shear dispersion techniques and flaxseed oil as green solvent (green biorefinery approach). Various combinations of time and temperature were used and final selection was made on the basis of maximum recovery of carotenoids. High shear disperser yielded maximum carotenoids (recovery 94.8 ± 0.08%). The total carotenoid content, antioxidant activity as ABTS, DDPH and FRAP and β-carotene of carotenoid rich extract from carrot pomace (CREP) were 82.66 ± 0.06 μg/g, 1596.04 ± 69.45 μg Trolox eq./ml, 380.21 ± 39.62 μg Trolox eq./ml, 941.20 ± 19.91 μM Trolox eq./ml, 78.37 μg/g, respectively were significantly higher ( p 
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In present study, carotenoids were extracted from carrot pomace using ultrasonication and high shear dispersion techniques and flaxseed oil as green solvent (green biorefinery approach). Various combinations of time and temperature were used and final selection was made on the basis of maximum recovery of carotenoids. High shear disperser yielded maximum carotenoids (recovery 94.8 ± 0.08%). The total carotenoid content, antioxidant activity as ABTS, DDPH and FRAP and β-carotene of carotenoid rich extract from carrot pomace (CREP) were 82.66 ± 0.06 μg/g, 1596.04 ± 69.45 μg Trolox eq./ml, 380.21 ± 39.62 μg Trolox eq./ml, 941.20 ± 19.91 μM Trolox eq./ml, 78.37 μg/g, respectively were significantly higher ( p  &lt; 0.05) when compared with the extracting medium. The L*, a* and b* values of CRE were 18.65 ± 0.037, 19.42 ± 0.21, 27.947 ± 0.65 and were significantly higher than extracting medium. The CRE could be used as a natural source of β-carotene and natural colorant for food applications.</description><identifier>ISSN: 0022-1155</identifier><identifier>EISSN: 0975-8402</identifier><identifier>DOI: 10.1007/s13197-019-03920-5</identifier><identifier>PMID: 31686699</identifier><language>eng</language><publisher>New Delhi: Springer India</publisher><subject>antioxidant activity ; Antioxidants ; beta-carotene ; Biorefineries ; biorefining ; Carotene ; Carotenoids ; carrots ; Chemical properties ; Chemistry ; Chemistry and Materials Science ; Chemistry/Food Science ; Dispersion ; dyes ; Flaxseed oil ; Food industry ; Food processing industry ; Food Science ; Food sources ; linseed oil ; Nutrition ; Organic chemistry ; Original ; Original Article ; Physicochemical properties ; pomace ; Recovery ; Seeds ; Solvents ; temperature ; ultrasonic treatment ; Vitamin E ; wastes ; β-Carotene</subject><ispartof>Journal of food science and technology, 2019-10, Vol.56 (10), p.4678-4687</ispartof><rights>Association of Food Scientists &amp; Technologists (India) 2019</rights><rights>Journal of Food Science and Technology is a copyright of Springer, (2019). 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In present study, carotenoids were extracted from carrot pomace using ultrasonication and high shear dispersion techniques and flaxseed oil as green solvent (green biorefinery approach). Various combinations of time and temperature were used and final selection was made on the basis of maximum recovery of carotenoids. High shear disperser yielded maximum carotenoids (recovery 94.8 ± 0.08%). The total carotenoid content, antioxidant activity as ABTS, DDPH and FRAP and β-carotene of carotenoid rich extract from carrot pomace (CREP) were 82.66 ± 0.06 μg/g, 1596.04 ± 69.45 μg Trolox eq./ml, 380.21 ± 39.62 μg Trolox eq./ml, 941.20 ± 19.91 μM Trolox eq./ml, 78.37 μg/g, respectively were significantly higher ( p  &lt; 0.05) when compared with the extracting medium. The L*, a* and b* values of CRE were 18.65 ± 0.037, 19.42 ± 0.21, 27.947 ± 0.65 and were significantly higher than extracting medium. 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In present study, carotenoids were extracted from carrot pomace using ultrasonication and high shear dispersion techniques and flaxseed oil as green solvent (green biorefinery approach). Various combinations of time and temperature were used and final selection was made on the basis of maximum recovery of carotenoids. High shear disperser yielded maximum carotenoids (recovery 94.8 ± 0.08%). The total carotenoid content, antioxidant activity as ABTS, DDPH and FRAP and β-carotene of carotenoid rich extract from carrot pomace (CREP) were 82.66 ± 0.06 μg/g, 1596.04 ± 69.45 μg Trolox eq./ml, 380.21 ± 39.62 μg Trolox eq./ml, 941.20 ± 19.91 μM Trolox eq./ml, 78.37 μg/g, respectively were significantly higher ( p  &lt; 0.05) when compared with the extracting medium. The L*, a* and b* values of CRE were 18.65 ± 0.037, 19.42 ± 0.21, 27.947 ± 0.65 and were significantly higher than extracting medium. 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subjects antioxidant activity
Antioxidants
beta-carotene
Biorefineries
biorefining
Carotene
Carotenoids
carrots
Chemical properties
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Dispersion
dyes
Flaxseed oil
Food industry
Food processing industry
Food Science
Food sources
linseed oil
Nutrition
Organic chemistry
Original
Original Article
Physicochemical properties
pomace
Recovery
Seeds
Solvents
temperature
ultrasonic treatment
Vitamin E
wastes
β-Carotene
title Organic solvent-free extraction of carotenoids from carrot bio-waste and its physico-chemical properties
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