HLA-A, HLA-B, and HLA-DRB1 Allele and Haplotype Frequencies in Renal Transplant Candidates in a Population in Southern Brazil

• Published in: Journal of clinical laboratory analysis 2016-05, Vol.30 (3), p.258-265
• Main Authors: Saito, Patrícia Keiko, Yamakawa, Roger Haruki, Noguti, Erika Noda, Bedendo, Gustavo Borelli, Júnior, Waldir Veríssimo da Silva, Yamada, Sérgio Seiji, Borelli, Sueli Donizete
• Format: Article
• Language: English
• Subjects: Alleles; Antigens; Brazil; chronic; Ethnic Groups - genetics; Female; Gene Frequency - genetics; genetic; Genotype & phenotype; Haplotypes - genetics; HLA antigens; HLA-A Antigens - genetics; HLA-B Antigens - genetics; HLA-DRB1 Chains - genetics; Humans; kidney failure; kidney failure, chronic; Kidney Transplantation; Kidneys; Leukocytes; major histocompatibility complex; Male; Phenotype; polymorphism; polymorphism, genetic; Transplants & implants
• ISSN: 0887-8013; 1098-2825
• DOI: 10.1002/jcla.21846

Background Very few studies have examined the diversity of human leukocyte antigens (HLA) in the Brazilian renal transplant candidates. Methods The frequencies of the HLA‐A, HLA‐B, and HLA‐DRB1 alleles, haplotypes and phenotypes were studied in 522 patients with chronic renal failure, renal transplant candidates, registered at the Transplant Centers in north/northwestern Paraná State, southern Brazil. Patients were classified according to the ethnic group (319 whites [Caucasians], 134 mestizos [mixed race descendants of Europeans, Africans, and Amerindians; browns or “pardos”] and 69 blacks). The HLA typing was performed by the polymerase chain reaction sequence‐specific oligonucleotide method (PCR‐SSO), combined with Luminex technology. Results In the analysis of the total samples, 20 HLA‐A, 32 HLA‐B, and 13 HLA‐DRB1 allele groups were identified. The most frequent allele groups for each HLA locus were HLA‐A*02 (25.4%), HLA‐B*44 (10.9%), and HLA‐DRB1*13 (13.9%). The most frequent haplotypes were HLA‐A*01‐B*08‐DRB1*03 (2.3%), A*02‐B*44‐DRB1*07 (1.2%), and A*03‐B*07‐DRB1*11 (1.0%). Significant differences (P < 0.05) were observed in the HLA‐A*68, B*08, and B*58 allele frequencies among ethnic groups. Conclusions This study provides the first data on the HLA‐A, HLA‐B, and HLA‐DRB1 allele, phenotype and haplotype frequencies of renal transplant candidates in a population in southern Brazil.