Use of gel microcolumn assay for the detection of drug-induced positive direct antiglobulin tests

Drugs can result in broad variety of hematologic abnormalities including positive direct antiglobulin test. In this study, we evaluated gel microcolumn assay for the detection of drug‐induced antibodies. Direct antiglobulin test was performed by conventional tube and by gel microcolumn assay in 139...

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Bibliographic Details
Published in:Journal of clinical laboratory analysis 2005, Vol.19 (5), p.219-227
Main Authors: Novaretti, M.C.Z., Sopeleti, C.R., Dorlhiac-Llacer, P.E., Chamone, D.A.F.
Format: Article
Language:English
Subjects:
Adsorption
Adult
Antigen-Antibody Complex - analysis
antiglobulin
Blood Donors
Chromatography, Gel
Coombs test
Coombs Test - instrumentation
Coombs Test - methods
Drug-Related Side Effects and Adverse Reactions
drugs
drugs adverse effects
Female
gel assay
gel test
Humans
Male
Middle Aged
Original
Prospective Studies
Sensitivity and Specificity
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Summary:Drugs can result in broad variety of hematologic abnormalities including positive direct antiglobulin test. In this study, we evaluated gel microcolumn assay for the detection of drug‐induced antibodies. Direct antiglobulin test was performed by conventional tube and by gel microcolumn assay in 139 hospitalized patients. Drug in vitro studies were done in 34 patients with positive direct antiglobulin test by tube test and gel microcolumn assay using serum and eluate. None of them had signs of hemolytic anemia. A total of 1,000 blood samples from donors were used as control group. Gel microcolumn assay was more sensitive than in tube test for direct antiglobulin test (P
ISSN:0887-8013
1098-2825
DOI:10.1002/jcla.20081