The cytokine alterations/abnormalities and oxidative damage in the pancreas during hypertension development

The aim of the present study was to compare the content of cytokines, chemokines, and oxidative stress markers in the pancreas of spontaneously hypertensive rats (SHRs) and Wistar Kyoto Rats (WKYs) serving as controls. Enzyme-like immunosorbent assay (ELISA) and biochemical methods were used to meas...

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Bibliographic Details
Published in:Pflügers Archiv 2019-10, Vol.471 (10), p.1331-1340
Main Authors: Kozłowska, Anna, Wojtacha, Paweł, Majewski, Michał, Równiak, Maciej
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Biomedicine
Cell Biology
Chemokines
Cytokines
Enzyme-linked immunosorbent assay
Human Physiology
Hypertension
Interferon
Interleukin 6
Malondialdehyde
Molecular Medicine
Monocyte chemoattractant protein
Monocyte chemoattractant protein 1
Monocytes
Neurosciences
Organ Physiology
Oxidation
Oxidative stress
Pancreas
RANTES
Receptors
Sulfhydryl groups
Transforming growth factor-b
Tumor necrosis factor-α
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Summary:The aim of the present study was to compare the content of cytokines, chemokines, and oxidative stress markers in the pancreas of spontaneously hypertensive rats (SHRs) and Wistar Kyoto Rats (WKYs) serving as controls. Enzyme-like immunosorbent assay (ELISA) and biochemical methods were used to measure pancreatic levels of interleukin-1ß, interleukin-6, tumor necrosis factor α, transforming growth factor β, RANES, monocyte chemoattractant protein 1, interferon gamma-induced protein 10, malondialdehyde, and sulfhydryl groups. The results showed that the pancreatic concentrations of all studied cytokines and chemokines did not differ between 5-week-old SHRs and WKYs, except RANTES which was significantly reduced in juvenile SHRs. In 10-week-old animals, except interleukin-1ß, the levels of all these proteins were significantly reduced in SHRs. The pancreatic levels of malondialdehyde were significantly reduced in 5-week-old SHRs and significantly elevated in 10-week-old SHRs while the contents of sulfhydryl groups were similar in both rat strains at any age studied. In conclusion, these data provide evidence that in maturating SHRs, the pancreatic levels of cytokines and chemokines are significantly reduced, while malondialdehyde significantly elevated. This suggests that in the pancreas of mature SHRs, the inflammation process is suppressed but there is ongoing oxidative damage.
ISSN:0031-6768
1432-2013
DOI:10.1007/s00424-019-02312-0