Resistin polymorphims, plasma resistin levels and obesity in Tunisian volunteers

• Published in: Journal of clinical laboratory analysis 2018-02, Vol.32 (2), p.n/a
• Main Authors: Zayani, Nesrine, Hamdouni, Haithem, Boumaiza, Imen, Achour, Ons, Neffati, Fadoua, Omezzine, Asma, Najjar, Mohamed Fadhel, Bouslama, Ali
• Format: Article
• Language: English
• Subjects: Adipocytes; Adipose tissue; Adult; Blood glucose; Body mass index; Diabetes mellitus; Enzyme-linked immunosorbent assay; Female; Gene polymorphism; Genotyping; Haplotypes; Homeostasis; Humans; Insulin; Leptin; levels; Male; Middle Aged; Obesity; Obesity - blood; Obesity - epidemiology; Obesity - genetics; Polymerase chain reaction; Polymorphism; Polymorphism, Single Nucleotide - genetics; polymorphisms; resistin; Resistin - blood; Resistin - genetics; Restriction fragment length polymorphism; Risk Factors; ROC Curve; Single-nucleotide polymorphism; Statistical analysis; Tunisia - epidemiology; tunisian volunteers
• ISSN: 0887-8013; 1098-2825
• DOI: 10.1002/jcla.22227

Background Adipose tissue is an important endocrine organ that secretes a number of adipokines, like Resistin (RETN); it's an adipocytes‐secreted cytokine and has been proposed as a link between obesity and diabetes. Many resistin gene polymorphisms were described and their implication in obesity was controversial. This study was to investigate the prevalence of single nucleotide polymorphisms (SNPs) in RETN gene 420C/G; 44G/A; 62G/A; 394C/G and 299 G/A and their association with Resistin level and obesity in Tunisian volunteers. Methods We recruited 169 nonobese (mean age=42.16‐14.26 years; mean body mass index [BMI]=24.51‐3.69 kg/m2) and 160 obese (mean age=47.86‐11.17 years; mean BMI=36‐4.78 kg/m2). Genotyping was performed using polymerase chain reaction–restriction fragment length polymorphism. Anthropometric parameters, lipid levels, Glycemia and insulinemia were measured, BMI was calculated and insulinresistance was evaluated with the homeostasis model assessment insulin resistance (HOMA‐IR) and resistin level was measured by ELISA. Statistical analyses were performed by SPSS19.0. Results After adjustment for confounding parameters; the Odds Ratio (OR) of obesity associated with mutated genotypes at 420C/G compared with normal genotype was as: OR=2.17; 95% CI [1.28‐3.68], P=.004. The serum Resistin levels present no significant association with all RETN polymorphisms and it was significantly associated with BMI (P=.047). In our haplotype analysis, one haplotype seems to be protective and one other seems to be the highest risk to obesity. Conclusion The 420 C/G Polymorphism were associated with obesity and Leptin concentration in our population.