More significance of TB‐IGRA except for the diagnose of tuberculosis

• Published in: Journal of clinical laboratory analysis 2018-01, Vol.32 (1), p.n/a
• Main Authors: Xu, Jun‐Chi, Li, Ze‐Yi, Chen, Xin‐Nian, Shi, Cui‐Lin, Wu, Mei‐Ying, Chen, Hui, Zhu, Xiao‐Yan, Song, Hua‐Feng, Wu, Min‐Juan, Xu, Ping
• Format: Article
• Language: English
• Subjects: Adult; CD28 antigen; CD4 antigen; CD4-Positive T-Lymphocytes - immunology; CD8 antigen; Female; Humans; Immunoassay; Interferon; Interferon-gamma - analysis; Interferon-gamma - immunology; Interferon-gamma - metabolism; Interferon-gamma Release Tests - statistics & numerical data; Leukocytes, Mononuclear - immunology; Lungs; Lymphocytes; Lymphocytes T; Male; Medical diagnosis; Middle Aged; Mycobacterium tuberculosis; Prognosis; Pulmonary lesions; Retrospective Studies; TB‐IGRA; Tuberculosis; Tuberculosis - diagnosis; Tuberculosis - epidemiology; Tuberculosis - immunology; γ‐interferon
• ISSN: 0887-8013; 1098-2825; 1098-2825
• DOI: 10.1002/jcla.22183

Objective Tuberculosis (TB)‐interferon gamma release assay (IGRA) test has the characteristics of short time, high specificity, and high sensitivity, but it lacks the correlation research between TB‐IGRA test results and body's immune cells, disease progression and prognosis, which is explored in this study. Design A retrospective study was carried out on positive TB‐IGRA patients who were infected with TB and diagnosed at our hospital from January 2014 to June 2015. The TB‐IGRA, routine blood test, T‐cell subgroup data were collected for statistical analysis. Results TB‐IGRA results were in positive proportion to the lymphocytes, CD4+T cells and CD4+CD28+T cells, whereas negative to the Treg cells. Patient with unilateral pulmonary lesion had higher TB‐IGRA than those with bilateral pulmonary lesions. After the stimulation of TB‐specific antigen, the proportion of CD4+IFN‐γ+ and CD8+IFN‐γ+T Tcells were both increased and the CD4+IFN‐γ+T had positive correlation with the value of TB‐IGRA. Conclusions IFN‐γ was tested with TB‐IGRA in patients with TB by the specific TB T cells and correlated with the lymphocytes, while the lymphocytes also closely related to the host's anti‐TB immunity and disease outcome. Hence the result of TB‐IGRA could reflect the specific anti‐TB immunity ability of the host, disease progression and prognosis. This study further expands the application scope of TB‐IGRA technology in the diagnosis of TB and lays a foundation for clinical practice to understand the immunity state of the patients with TB and the application of auxiliary clinical immunity regulators.