Longitudinal micro-endoscopic monitoring of high-success intramucosal xenografts for mouse models of colorectal cancer

Colorectal cancer (CRC) is one of the most frequently lethal forms of cancer. Intramucosal injection allows development of better mouse models of CRC, as orthotopic xenografts allow development of adenocarcinoma in the submucosa of the mouse colon wall. In this paper, a method of orthotopic injectio...

Full description

Saved in:
Bibliographic Details
Published in:International journal of medical sciences 2019-01, Vol.16 (11), p.1453-1460
Main Authors: Paulson, Bjorn, Kim, Ick Hee, Namgoong, Jung-Man, Kim, Young Gyu, Lee, Sanghwa, Moon, Youngjin, Shin, Dong-Myung, Choo, Myung-Soo, Kim, Jun Ki
Format: Article
Language:English
Subjects:
Adenocarcinoma - pathology
Adenoma - pathology
Animals
Colon - pathology
Colonoscopy
Colorectal cancer
Colorectal Neoplasms - pathology
Disease Models, Animal
Drug efficacy
Endoscopy
HCT116 Cells
Heterografts - pathology
Humans
Laboratory animals
Lasers
Mice
Mortality
Proteins
Research Paper
Success
Tumors
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Colorectal cancer (CRC) is one of the most frequently lethal forms of cancer. Intramucosal injection allows development of better mouse models of CRC, as orthotopic xenografts allow development of adenocarcinoma in the submucosa of the mouse colon wall. In this paper, a method of orthotopic injection is monitored longitudinally using cellular-resolution real-time fluorescence microendoscopy, following the injection of three different cell lines: 3T3-GFP to confirm immunosuppression and HCT116-RFP cells to model CRC. Adenoma formation is first observable after 7 to 10 days, and by use of 33 G needles a tumor induction rate of greater than 85% is documented. An additional experiment on the injection of rapamycin reveals drug efficacy and localization between 24 and 48 hours, and suggests the promise of real-time cellular-resolution fluorescence micro-endoscopy for developing longitudinal therapy regimes in mural models of CRC.
ISSN:1449-1907
1449-1907
DOI:10.7150/ijms.35666