Hsa_circ_0000467 promotes cancer progression and serves as a diagnostic and prognostic biomarker for gastric cancer

• Published in: Journal of clinical laboratory analysis 2019-03, Vol.33 (3), p.e22726-n/a
• Main Authors: Lu, Jun, Zhang, Peng‐yang, Xie, Jian‐wei, Wang, Jia‐bin, Lin, Jian‐xian, Chen, Qi‐yue, Cao, Long‐long, Huang, Chang‐ming, Li, Ping, Zheng, Chao‐hui
• Format: Article
• Language: English
• Subjects: Apoptosis; biomarker; Biomarkers; Biomarkers, Tumor - analysis; Biomarkers, Tumor - genetics; Biomarkers, Tumor - metabolism; carcinogenesis; Case-Control Studies; Cell Line, Tumor; Cell Proliferation; circular RNA; Disease Progression; Gastric cancer; hsa_circ_0000467; Humans; Middle Aged; Multivariate analysis; Organ Specificity; Polymerase chain reaction; Prognosis; Reverse transcription; RNA - analysis; RNA - genetics; RNA - metabolism; RNA, Circular; ROC Curve; Stomach Neoplasms - genetics; Stomach Neoplasms - metabolism; Stomach Neoplasms - pathology; Surgery; Therapeutic applications
• ISSN: 0887-8013; 1098-2825; 1098-2825
• DOI: 10.1002/jcla.22726

Background Emerging evidence indicates that dysregulation of circular RNAs (circRNAs) is implicated in the development of malignancies. However, the diagnostic value and functional role of circRNAs in gastric cancer (GC) remain largely elusive. Methods Quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR) was performed to detect the expression of hsa_circ_0000467 in tissues, cell lines, and plasma. A receiver operating characteristic (ROC) curve was constructed to estimate the diagnostic value of hsa_circ_0000467. The association between the expression level of hsa_circ_0000467 and the clinicopathological features was analyzed. Moreover, cell functional assays were performed. Results Hsa_circ_0000467 was significantly upregulated in GC tissue compared to adjacent nontumor tissue (n = 51, P