Loading…
Distinguish metabolic profiles and defense enzymes in Alternaria leaf blight resistant and susceptible genotypes of groundnut
Alternaria leaf blight is major fungal disease of summer groundnut, causes significant loss of haulm and pod yield. Aims of this study were to understand the role of metabolites and phenylpropanoid related enzymes in Alternaria leaf blight resistance and to find out metabolic marker for disease resi...
Saved in:
| Published in: | Physiology and molecular biology of plants 2019-11, Vol.25 (6), p.1395-1405 |
|---|---|
| Main Authors: | , , , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c451t-90594fb7dd920e8852618fe89247be57d94c5df2d699b511bd8a364a199acc2e3 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c451t-90594fb7dd920e8852618fe89247be57d94c5df2d699b511bd8a364a199acc2e3 |
| container_end_page | 1405 |
| container_issue | 6 |
| container_start_page | 1395 |
| container_title | Physiology and molecular biology of plants |
| container_volume | 25 |
| creator | Mahatma, M. K. Thawait, L. K. Jadon, K. S. Rathod, K. J. Sodha, K. H. Bishi, S. K. Thirumalaisamy, P. P. Golakiya, B. A. |
| description | Alternaria leaf blight is major fungal disease of summer groundnut, causes significant loss of haulm and pod yield. Aims of this study were to understand the role of metabolites and phenylpropanoid related enzymes in
Alternaria
leaf blight resistance and to find out metabolic marker for disease resistance.
Alternaria
leaf blight resistant (GPBD4 and CS186) and susceptible genotypes (GG2 and TPG41) of groundnut were grown in pots during
rabi
-summer 2015. Groundnut plants were infected with
Alternaria alternata
(Fr.)
Keissler
at 40 days after sowing. 5 days after infection, upper second leaves were collected from both control and infected plants for analysis. A total of 67 metabolites comprising sugars, sugar alcohols, amino acids, organic acids, fatty acids, sterols and phenolic were identified using gas chromatography–mass spectrometry (non-targeted metabolomics). Constitutive levels of alpha-
d
-galactoside,
d
-mannitol,
d
-erythropentitol, glycine, and hexadecanoic acid were observed higher in resistant genotypes compared to susceptible genotypes. Moreover, arabinofuranose, cinnamic acid, 2-butendioic acid, and linoleic acid were observed only in resistant genotypes at both control and infected stage. In susceptible genotypes
myo
-inositol, glucose and fructose content was increased after infection with pathogen while decreased in resistant genotypes. Resistant genotypes had higher constitutive level of cinnamic and salicylic acid compared to susceptible genotypes. Non-infected leaves of resistant genotypes also had higher activities of phenylalanine ammonia lyase and tyrosine ammonia lyase activities. Our results suggest that metabolites specifically present in resistant genotypes impart defense mechanism against
Alternaria
pathogen and can be used as bio-marker for screening of germplasm. |
| doi_str_mv | 10.1007/s12298-019-00708-x |
| format | article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6825051</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2315527584</sourcerecordid><originalsourceid>FETCH-LOGICAL-c451t-90594fb7dd920e8852618fe89247be57d94c5df2d699b511bd8a364a199acc2e3</originalsourceid><addsrcrecordid>eNp9kUtrFTEYhgex2Fr9A64CbtyM5jqTbITSeoNCN7oOmck3c1IyyZhkpEfwv5ueUxRddJXb87wkeZvmFcFvCcb9u0woVbLFRLV1iWV796Q5w6rnLeYMPz3MSSuk4qfN85xvMe4Y78mz5pSRnnWCs7Pm15XLxYV5c3mHFihmiN6NaE1xch4yMsEiCxOEDAjCz_1S91xAF75ACiY5gzyYCQ3ezbuCEuQaZ0I5eHnLI6zFDR7QDCGW_VrtOKE5xS3YsJUXzclkfIaXD-N58-3jh6-Xn9vrm09fLi-u25ELUlqFheLT0FurKAYpBe2InEAqyvsBRG8VH4WdqO2UGgQhg5WGddwQpcw4UmDnzftj7roNC9gRQknG6zW5xaS9jsbpf0-C2-k5_tCdpAILUgPePASk-H2DXPTi6uO8NwHiljVlRAjaC8kr-vo_9DZu9a_8gSKUMcX6StEjNaaYc4Lpz2UI1vft6mO7urarD-3quyqxo5QrHGZIf6MfsX4DS1-qdQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2311233937</pqid></control><display><type>article</type><title>Distinguish metabolic profiles and defense enzymes in Alternaria leaf blight resistant and susceptible genotypes of groundnut</title><source>Springer Link</source><source>PubMed Central</source><creator>Mahatma, M. K. ; Thawait, L. K. ; Jadon, K. S. ; Rathod, K. J. ; Sodha, K. H. ; Bishi, S. K. ; Thirumalaisamy, P. P. ; Golakiya, B. A.</creator><creatorcontrib>Mahatma, M. K. ; Thawait, L. K. ; Jadon, K. S. ; Rathod, K. J. ; Sodha, K. H. ; Bishi, S. K. ; Thirumalaisamy, P. P. ; Golakiya, B. A.</creatorcontrib><description>Alternaria leaf blight is major fungal disease of summer groundnut, causes significant loss of haulm and pod yield. Aims of this study were to understand the role of metabolites and phenylpropanoid related enzymes in
Alternaria
leaf blight resistance and to find out metabolic marker for disease resistance.
Alternaria
leaf blight resistant (GPBD4 and CS186) and susceptible genotypes (GG2 and TPG41) of groundnut were grown in pots during
rabi
-summer 2015. Groundnut plants were infected with
Alternaria alternata
(Fr.)
Keissler
at 40 days after sowing. 5 days after infection, upper second leaves were collected from both control and infected plants for analysis. A total of 67 metabolites comprising sugars, sugar alcohols, amino acids, organic acids, fatty acids, sterols and phenolic were identified using gas chromatography–mass spectrometry (non-targeted metabolomics). Constitutive levels of alpha-
d
-galactoside,
d
-mannitol,
d
-erythropentitol, glycine, and hexadecanoic acid were observed higher in resistant genotypes compared to susceptible genotypes. Moreover, arabinofuranose, cinnamic acid, 2-butendioic acid, and linoleic acid were observed only in resistant genotypes at both control and infected stage. In susceptible genotypes
myo
-inositol, glucose and fructose content was increased after infection with pathogen while decreased in resistant genotypes. Resistant genotypes had higher constitutive level of cinnamic and salicylic acid compared to susceptible genotypes. Non-infected leaves of resistant genotypes also had higher activities of phenylalanine ammonia lyase and tyrosine ammonia lyase activities. Our results suggest that metabolites specifically present in resistant genotypes impart defense mechanism against
Alternaria
pathogen and can be used as bio-marker for screening of germplasm.</description><identifier>ISSN: 0971-5894</identifier><identifier>EISSN: 0974-0430</identifier><identifier>DOI: 10.1007/s12298-019-00708-x</identifier><identifier>PMID: 31736543</identifier><language>eng</language><publisher>New Delhi: Springer India</publisher><subject>Alcohols ; Alternaria ; Amino acids ; Ammonia ; Biological and Medical Physics ; Biomarkers ; Biomedical and Life Sciences ; Biophysics ; Blight ; Cell Biology ; Cinnamic acid ; Disease resistance ; Enzymes ; Fatty acids ; Fructose ; Fungal diseases ; Galactosides ; Gas chromatography ; Genotypes ; Germplasm ; Glycine ; Groundnuts ; Infections ; Inositol ; Leaf blight ; Leaves ; Life Sciences ; Linoleic acid ; Mannitol ; Mass spectrometry ; Mass spectroscopy ; Metabolism ; Metabolites ; Metabolomics ; Organic acids ; Palmitic acid ; Pathogens ; Phenols ; Phenylalanine ; Plant Physiology ; Plant Sciences ; Research Article ; Salicylic acid ; Sterols ; Sugar ; Summer ; Tyrosine</subject><ispartof>Physiology and molecular biology of plants, 2019-11, Vol.25 (6), p.1395-1405</ispartof><rights>Prof. H.S. Srivastava Foundation for Science and Society 2019</rights><rights>Copyright Springer Nature B.V. 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c451t-90594fb7dd920e8852618fe89247be57d94c5df2d699b511bd8a364a199acc2e3</citedby><cites>FETCH-LOGICAL-c451t-90594fb7dd920e8852618fe89247be57d94c5df2d699b511bd8a364a199acc2e3</cites><orcidid>0000-0002-9596-0076</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6825051/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6825051/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids></links><search><creatorcontrib>Mahatma, M. K.</creatorcontrib><creatorcontrib>Thawait, L. K.</creatorcontrib><creatorcontrib>Jadon, K. S.</creatorcontrib><creatorcontrib>Rathod, K. J.</creatorcontrib><creatorcontrib>Sodha, K. H.</creatorcontrib><creatorcontrib>Bishi, S. K.</creatorcontrib><creatorcontrib>Thirumalaisamy, P. P.</creatorcontrib><creatorcontrib>Golakiya, B. A.</creatorcontrib><title>Distinguish metabolic profiles and defense enzymes in Alternaria leaf blight resistant and susceptible genotypes of groundnut</title><title>Physiology and molecular biology of plants</title><addtitle>Physiol Mol Biol Plants</addtitle><description>Alternaria leaf blight is major fungal disease of summer groundnut, causes significant loss of haulm and pod yield. Aims of this study were to understand the role of metabolites and phenylpropanoid related enzymes in
Alternaria
leaf blight resistance and to find out metabolic marker for disease resistance.
Alternaria
leaf blight resistant (GPBD4 and CS186) and susceptible genotypes (GG2 and TPG41) of groundnut were grown in pots during
rabi
-summer 2015. Groundnut plants were infected with
Alternaria alternata
(Fr.)
Keissler
at 40 days after sowing. 5 days after infection, upper second leaves were collected from both control and infected plants for analysis. A total of 67 metabolites comprising sugars, sugar alcohols, amino acids, organic acids, fatty acids, sterols and phenolic were identified using gas chromatography–mass spectrometry (non-targeted metabolomics). Constitutive levels of alpha-
d
-galactoside,
d
-mannitol,
d
-erythropentitol, glycine, and hexadecanoic acid were observed higher in resistant genotypes compared to susceptible genotypes. Moreover, arabinofuranose, cinnamic acid, 2-butendioic acid, and linoleic acid were observed only in resistant genotypes at both control and infected stage. In susceptible genotypes
myo
-inositol, glucose and fructose content was increased after infection with pathogen while decreased in resistant genotypes. Resistant genotypes had higher constitutive level of cinnamic and salicylic acid compared to susceptible genotypes. Non-infected leaves of resistant genotypes also had higher activities of phenylalanine ammonia lyase and tyrosine ammonia lyase activities. Our results suggest that metabolites specifically present in resistant genotypes impart defense mechanism against
Alternaria
pathogen and can be used as bio-marker for screening of germplasm.</description><subject>Alcohols</subject><subject>Alternaria</subject><subject>Amino acids</subject><subject>Ammonia</subject><subject>Biological and Medical Physics</subject><subject>Biomarkers</subject><subject>Biomedical and Life Sciences</subject><subject>Biophysics</subject><subject>Blight</subject><subject>Cell Biology</subject><subject>Cinnamic acid</subject><subject>Disease resistance</subject><subject>Enzymes</subject><subject>Fatty acids</subject><subject>Fructose</subject><subject>Fungal diseases</subject><subject>Galactosides</subject><subject>Gas chromatography</subject><subject>Genotypes</subject><subject>Germplasm</subject><subject>Glycine</subject><subject>Groundnuts</subject><subject>Infections</subject><subject>Inositol</subject><subject>Leaf blight</subject><subject>Leaves</subject><subject>Life Sciences</subject><subject>Linoleic acid</subject><subject>Mannitol</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Metabolism</subject><subject>Metabolites</subject><subject>Metabolomics</subject><subject>Organic acids</subject><subject>Palmitic acid</subject><subject>Pathogens</subject><subject>Phenols</subject><subject>Phenylalanine</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Research Article</subject><subject>Salicylic acid</subject><subject>Sterols</subject><subject>Sugar</subject><subject>Summer</subject><subject>Tyrosine</subject><issn>0971-5894</issn><issn>0974-0430</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp9kUtrFTEYhgex2Fr9A64CbtyM5jqTbITSeoNCN7oOmck3c1IyyZhkpEfwv5ueUxRddJXb87wkeZvmFcFvCcb9u0woVbLFRLV1iWV796Q5w6rnLeYMPz3MSSuk4qfN85xvMe4Y78mz5pSRnnWCs7Pm15XLxYV5c3mHFihmiN6NaE1xch4yMsEiCxOEDAjCz_1S91xAF75ACiY5gzyYCQ3ezbuCEuQaZ0I5eHnLI6zFDR7QDCGW_VrtOKE5xS3YsJUXzclkfIaXD-N58-3jh6-Xn9vrm09fLi-u25ELUlqFheLT0FurKAYpBe2InEAqyvsBRG8VH4WdqO2UGgQhg5WGddwQpcw4UmDnzftj7roNC9gRQknG6zW5xaS9jsbpf0-C2-k5_tCdpAILUgPePASk-H2DXPTi6uO8NwHiljVlRAjaC8kr-vo_9DZu9a_8gSKUMcX6StEjNaaYc4Lpz2UI1vft6mO7urarD-3quyqxo5QrHGZIf6MfsX4DS1-qdQ</recordid><startdate>20191101</startdate><enddate>20191101</enddate><creator>Mahatma, M. K.</creator><creator>Thawait, L. K.</creator><creator>Jadon, K. S.</creator><creator>Rathod, K. J.</creator><creator>Sodha, K. H.</creator><creator>Bishi, S. K.</creator><creator>Thirumalaisamy, P. P.</creator><creator>Golakiya, B. A.</creator><general>Springer India</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-9596-0076</orcidid></search><sort><creationdate>20191101</creationdate><title>Distinguish metabolic profiles and defense enzymes in Alternaria leaf blight resistant and susceptible genotypes of groundnut</title><author>Mahatma, M. K. ; Thawait, L. K. ; Jadon, K. S. ; Rathod, K. J. ; Sodha, K. H. ; Bishi, S. K. ; Thirumalaisamy, P. P. ; Golakiya, B. A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c451t-90594fb7dd920e8852618fe89247be57d94c5df2d699b511bd8a364a199acc2e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Alcohols</topic><topic>Alternaria</topic><topic>Amino acids</topic><topic>Ammonia</topic><topic>Biological and Medical Physics</topic><topic>Biomarkers</topic><topic>Biomedical and Life Sciences</topic><topic>Biophysics</topic><topic>Blight</topic><topic>Cell Biology</topic><topic>Cinnamic acid</topic><topic>Disease resistance</topic><topic>Enzymes</topic><topic>Fatty acids</topic><topic>Fructose</topic><topic>Fungal diseases</topic><topic>Galactosides</topic><topic>Gas chromatography</topic><topic>Genotypes</topic><topic>Germplasm</topic><topic>Glycine</topic><topic>Groundnuts</topic><topic>Infections</topic><topic>Inositol</topic><topic>Leaf blight</topic><topic>Leaves</topic><topic>Life Sciences</topic><topic>Linoleic acid</topic><topic>Mannitol</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Metabolism</topic><topic>Metabolites</topic><topic>Metabolomics</topic><topic>Organic acids</topic><topic>Palmitic acid</topic><topic>Pathogens</topic><topic>Phenols</topic><topic>Phenylalanine</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Research Article</topic><topic>Salicylic acid</topic><topic>Sterols</topic><topic>Sugar</topic><topic>Summer</topic><topic>Tyrosine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mahatma, M. K.</creatorcontrib><creatorcontrib>Thawait, L. K.</creatorcontrib><creatorcontrib>Jadon, K. S.</creatorcontrib><creatorcontrib>Rathod, K. J.</creatorcontrib><creatorcontrib>Sodha, K. H.</creatorcontrib><creatorcontrib>Bishi, S. K.</creatorcontrib><creatorcontrib>Thirumalaisamy, P. P.</creatorcontrib><creatorcontrib>Golakiya, B. A.</creatorcontrib><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Physiology and molecular biology of plants</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mahatma, M. K.</au><au>Thawait, L. K.</au><au>Jadon, K. S.</au><au>Rathod, K. J.</au><au>Sodha, K. H.</au><au>Bishi, S. K.</au><au>Thirumalaisamy, P. P.</au><au>Golakiya, B. A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Distinguish metabolic profiles and defense enzymes in Alternaria leaf blight resistant and susceptible genotypes of groundnut</atitle><jtitle>Physiology and molecular biology of plants</jtitle><stitle>Physiol Mol Biol Plants</stitle><date>2019-11-01</date><risdate>2019</risdate><volume>25</volume><issue>6</issue><spage>1395</spage><epage>1405</epage><pages>1395-1405</pages><issn>0971-5894</issn><eissn>0974-0430</eissn><abstract>Alternaria leaf blight is major fungal disease of summer groundnut, causes significant loss of haulm and pod yield. Aims of this study were to understand the role of metabolites and phenylpropanoid related enzymes in
Alternaria
leaf blight resistance and to find out metabolic marker for disease resistance.
Alternaria
leaf blight resistant (GPBD4 and CS186) and susceptible genotypes (GG2 and TPG41) of groundnut were grown in pots during
rabi
-summer 2015. Groundnut plants were infected with
Alternaria alternata
(Fr.)
Keissler
at 40 days after sowing. 5 days after infection, upper second leaves were collected from both control and infected plants for analysis. A total of 67 metabolites comprising sugars, sugar alcohols, amino acids, organic acids, fatty acids, sterols and phenolic were identified using gas chromatography–mass spectrometry (non-targeted metabolomics). Constitutive levels of alpha-
d
-galactoside,
d
-mannitol,
d
-erythropentitol, glycine, and hexadecanoic acid were observed higher in resistant genotypes compared to susceptible genotypes. Moreover, arabinofuranose, cinnamic acid, 2-butendioic acid, and linoleic acid were observed only in resistant genotypes at both control and infected stage. In susceptible genotypes
myo
-inositol, glucose and fructose content was increased after infection with pathogen while decreased in resistant genotypes. Resistant genotypes had higher constitutive level of cinnamic and salicylic acid compared to susceptible genotypes. Non-infected leaves of resistant genotypes also had higher activities of phenylalanine ammonia lyase and tyrosine ammonia lyase activities. Our results suggest that metabolites specifically present in resistant genotypes impart defense mechanism against
Alternaria
pathogen and can be used as bio-marker for screening of germplasm.</abstract><cop>New Delhi</cop><pub>Springer India</pub><pmid>31736543</pmid><doi>10.1007/s12298-019-00708-x</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-9596-0076</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0971-5894 |
| ispartof | Physiology and molecular biology of plants, 2019-11, Vol.25 (6), p.1395-1405 |
| issn | 0971-5894 0974-0430 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6825051 |
| source | Springer Link; PubMed Central |
| subjects | Alcohols Alternaria Amino acids Ammonia Biological and Medical Physics Biomarkers Biomedical and Life Sciences Biophysics Blight Cell Biology Cinnamic acid Disease resistance Enzymes Fatty acids Fructose Fungal diseases Galactosides Gas chromatography Genotypes Germplasm Glycine Groundnuts Infections Inositol Leaf blight Leaves Life Sciences Linoleic acid Mannitol Mass spectrometry Mass spectroscopy Metabolism Metabolites Metabolomics Organic acids Palmitic acid Pathogens Phenols Phenylalanine Plant Physiology Plant Sciences Research Article Salicylic acid Sterols Sugar Summer Tyrosine |
| title | Distinguish metabolic profiles and defense enzymes in Alternaria leaf blight resistant and susceptible genotypes of groundnut |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T20%3A22%3A04IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Distinguish%20metabolic%20profiles%20and%20defense%20enzymes%20in%20Alternaria%20leaf%20blight%20resistant%20and%20susceptible%20genotypes%20of%20groundnut&rft.jtitle=Physiology%20and%20molecular%20biology%20of%20plants&rft.au=Mahatma,%20M.%20K.&rft.date=2019-11-01&rft.volume=25&rft.issue=6&rft.spage=1395&rft.epage=1405&rft.pages=1395-1405&rft.issn=0971-5894&rft.eissn=0974-0430&rft_id=info:doi/10.1007/s12298-019-00708-x&rft_dat=%3Cproquest_pubme%3E2315527584%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c451t-90594fb7dd920e8852618fe89247be57d94c5df2d699b511bd8a364a199acc2e3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2311233937&rft_id=info:pmid/31736543&rfr_iscdi=true |