Expression of Irisin/FNDC5 in Cancer Cells and Stromal Fibroblasts of Non-Small Cell Lung Cancer

Background: Recent in vitro studies have indicated that irisin inhibits proliferation, migration and epithelial-mesenchymal transition. Irisin expression has not been studied in tumour tissues of non-small cell lung cancer (NSCLC) patients yet. The aim of the study was to determine the irisin expres...

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Published in:Cancers 2019-10, Vol.11 (10), p.1538
Main Authors: Nowinska, Katarzyna, Jablonska, Karolina, Pawelczyk, Konrad, Piotrowska, Aleksandra, Partynska, Aleksandra, Gomulkiewicz, Agnieszka, Ciesielska, Urszula, Katnik, Ewa, Grzegrzolka, Jedrzej, Glatzel-Plucinska, Natalia, Ratajczak-Wielgomas, Katarzyna, Podhorska-Okolow, Marzenna, Dziegiel, Piotr
Format: Article
Language:English
Subjects:
Antigens
Body temperature
Breast cancer
Cell division
Cell growth
Cell proliferation
Fibroblasts
Gene expression
Immunofluorescence
Lung cancer
Lymph nodes
Malignancy
Mesenchyme
Metastases
Musculoskeletal system
Non-small cell lung carcinoma
Polymerase chain reaction
Proteins
Small cell lung carcinoma
Stromal cells
Tumor cell lines
Tumors
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Summary:Background: Recent in vitro studies have indicated that irisin inhibits proliferation, migration and epithelial-mesenchymal transition. Irisin expression has not been studied in tumour tissues of non-small cell lung cancer (NSCLC) patients yet. The aim of the study was to determine the irisin expression in NSCLCs in comparison to the clinicopathological factors and expression of TTF-1, p63 and Ki-67. Material and methods: Tissue microarrays with 729 NSCLC and 140 non-malignant lung tissue (NMLT) were used to perform immunohistochemical reactions. Laser Capture Microdissection (LCM) was used to collect cancer and stromal cells from NSCLCs. FNDC5 expression was tested for LCM samples, 75 NSCLCs and 25 NMLTs with the RT-PCR technique. Western-blot, immunofluorescence reaction and RT-PCR assays were performed on lung cancer cell lines. Results: Irisin expression was observed in NSCLC cancer cells and stromal fibroblasts. In cancer cells, irisin expression was decreased in higher grades (G) of malignancy, tumour size (T) and according to lymph node metastasis. In stromal cells, irisin expression was increased in higher G and advanced T. A shorter overall survival was observed in patients with higher irisin expression in NSCLC stromal cells. Conclusions: Irisin expression in stromal fibroblasts may influence cancer cell proliferation and may be a prognostic factor for survival in NSCLC.
ISSN:2072-6694
2072-6694
DOI:10.3390/cancers11101538