Cocktail Strategy Based on NK Cell-Derived Exosomes and Their Biomimetic Nanoparticles for Dual Tumor Therapy

Successful cancer therapy requires drugs being precisely delivered to tumors. Nanosized drugs have attracted considerable recent attention, but their toxicity and high immunogenicity are important obstacles hampering their clinical translation. Here we report a novel “cocktail therapy” strategy base...

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Bibliographic Details
Published in:Cancers 2019-10, Vol.11 (10), p.1560
Main Authors: Wang, Guosheng, Hu, Weilei, Chen, Haiqiong, Shou, Xin, Ye, Tingting, Xu, Yibing
Format: Article
Language:English
Subjects:
Apoptosis
Biocompatibility
Cancer therapies
Confocal microscopy
Cytotoxicity
Drug delivery systems
Efficiency
Exosomes
Immunogenicity
MicroRNAs
Microscopy
miRNA
Nanoparticles
Natural killer cells
Neuroblastoma
Neuroblastoma cells
Polymers
Proteins
Quality control
Toxicity
Transmission electron microscopy
Tumors
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Summary:Successful cancer therapy requires drugs being precisely delivered to tumors. Nanosized drugs have attracted considerable recent attention, but their toxicity and high immunogenicity are important obstacles hampering their clinical translation. Here we report a novel “cocktail therapy” strategy based on excess natural killer cell-derived exosomes (NKEXOs) in combination with their biomimetic core–shell nanoparticles (NNs) for tumor-targeted therapy. The NNs were self- assembled with a dendrimer core loading therapeutic miRNA and a hydrophilic NKEXOs shell. Their successful fabrication was confirmed by transmission electron microscopy (TEM) and confocal laser scanning microscopy (CLSM). The resulting NN/NKEXO cocktail showed highly efficient targeting and therapeutic miRNA delivery to neuroblastoma cells in vivo, as demonstrated by two-photon excited scanning fluorescence imaging (TPEFI) and with an IVIS Spectrum in vivo imaging system (IVIS), leading to dual inhibition of tumor growth. With unique biocompatibility, we propose this NN/NKEXO cocktail as a new avenue for tumor therapy, with potential prospects for clinical applications.
ISSN:2072-6694
2072-6694
DOI:10.3390/cancers11101560