Oxidized base 8-oxoguanine, a product of DNA repair processes, contributes to dendritic cell activation

• Published in: Free radical biology & medicine 2019-11, Vol.143, p.209-220
• Main Authors: Pázmándi, Kitti, Sütő, Máté, Fekete, Tünde, Varga, Aliz, Boldizsár, Eszter, Boldogh, István, Bácsi, Attila
• Format: Article
• Language: English
• Subjects: 7,8-Dihydro-8-oxoguanine; 8-Oxoguanine DNA glycosylase 1; Dendritic cell activation; DNA base excision repair process
• ISSN: 0891-5849; 1873-4596
• DOI: 10.1016/j.freeradbiomed.2019.08.010

A growing body of evidence suggests that elevated levels of reactive oxygen species (ROS) in the airways caused by exposure to gas phase pollutants or particulate matter are able to activate dendritic cells (DCs); however, the exact mechanisms are still unclear. When present in excess, ROS can modify macromolecules including DNA. One of the most abundant DNA base lesions is 7,8-dihydro-8-oxoguanine (8-oxoG), which is repaired by the 8-oxoguanine DNA glycosylase 1 (OGG1)-initiated base excision repair (BER) (OGG1-BER) pathway. Studies have also demonstrated that in addition to its role in repairing oxidized purines, OGG1 has guanine nucleotide exchange factor activity when bound to 8-oxoG. In the present study, we tested the hypothesis that exposure to 8-oxoG, the specific product of OGG1-BER, induces functional changes of DCs. Supporting our hypothesis, transcriptome analysis revealed that in mouse lungs, out of 95 genes associated with DCs’ function, 22 or 42 were significantly upregulated after a single or multiple intranasal 8-oxoG challenges, respectively. In a murine model of allergic airway inflammation, significantly increased serum levels of ovalbumin (OVA)-specific IgE antibodies were detected in mice sensitized via nasal challenges with OVA+8-oxoG compared to those challenged with OVA alone. Furthermore, exposure of primary human monocyte-derived DCs (moDC) to 8-oxoG base resulted in significantly enhanced expression of cell surface molecules (CD40, CD86, CD83, HLA-DQ) and augmented the secretion of pro-inflammatory mediators IL-6, TNF and IL-8, whereas it did not considerably influence the production of the anti-inflammatory cytokine IL-10. The stimulatory effects of 8-oxoG on human moDCs were abolished upon siRNA-mediated OGG1 depletion. Collectively, these data suggest that OGG1-BER-generated 8-oxoG base-driven cell signaling activates DCs, which may contribute to initiation of both the innate and adaptive immune responses under conditions of oxidative stress. [Display omitted] •The most frequent oxidation product of guanine in DNA is 8-oxoG.•8-oxoG is removed from DNA as a base during OGG1-initiated BER.•OGG1 bound to the free 8-oxoG has guanine nucleotide exchange factor (GEF) activity.•8-oxoG base alters the expression of genes linked to DC activation in murine lungs.•8-oxoG base induces the activation of human moDCs in an OGG1-dependent manner.