Characterization of juice fermented with Lactobacillus plantarum EM and its cholesterol‐lowering effects on rats fed a high‐fat and high‐cholesterol diet

• Published in: Food science & nutrition 2019-11, Vol.7 (11), p.3622-3634
• Main Authors: Jeon, Yu Bin, Lee, Jae‐Joon, Chang, Hae Choon
• Format: Article
• Language: English
• Subjects: Antiinfectives and antibacterials; Antimicrobial activity; Assimilation; Cholesterol; Diet; Fatty acids; Fermentation; Food processing industry; Food safety; Fruit juices; Fungi; Gene expression; High cholesterol diet; High fat diet; Hydroxylase; Hypercholesterolemia; hypercholesterolemic rat; hypocholesterolemic effect; juice fermentation; Juices; Lactobacillus plantarum; Lactobacillus plantarum EM; Lipids; Low density lipoprotein; Metabolism; Microorganisms; Original Research; Polymerase chain reaction; Receptor density; Reductase; Reductases; Reverse transcription; starter culture; Starter cultures; Triglycerides
• ISSN: 2048-7177; 2048-7177
• DOI: 10.1002/fsn3.1217

The aim of this study was to investigate the ability of Lactobacillus plantarum EM as a starter culture to control cabbage–apple juice fermentation and to explore the cholesterol‐lowering effects of the fermented juice (EM juice) in rats. L. plantarum EM produced strong antimicrobial activities against bacteria and fungi, suppressing other microorganisms in the fermented juice, and was the dominant organism during fermentation and storage. The EM juice also showed strong and broad‐spectrum antimicrobial activity. Rats fed a high‐fat and high‐cholesterol diet and administered EM juice showed significantly reduced total cholesterol (TC), triglyceride, and LDL‐cholesterol levels, as well as a reduced atherogenic index, lower cardiac factors in serum, and lower TC levels in the liver, while total lipid and TC levels in the rat feces increased. Reverse transcription–polymerase chain reaction analysis revealed that the hepatic mRNA expression of HMG‐CoA reductase decreased and the expressions of cholesterol 7α‐hydroxylase and low‐density lipoprotein receptor increased in rats administered EM juice. The effects of EM juice on rats included inhibition of cholesterol synthesis as well as enhancement of cholesterol uptake and cholesterol excretion. The results of this study indicate that the use of L. plantarum EM as a functional starter culture for juice fermentation exerts microbial control, enhances sanitary safety, and provides beneficial food effects against hypercholesterolemia. The application of L. plantarum EM in juice fermentation as a functional starter culture exerts microbial control, sanitary safety as well as improved organoleptic quality, and beneficial food effects against hypercholesterolemia. The effects of the fermented EM juice on rats included inhibition of cholesterol synthesis and enhancement of cholesterol uptake along with high excretion of cholesterol.