Effect and mechanisms of sacral nerve stimulation on visceral hypersensitivity mediated by nerve growth factor

• Published in: Journal of cellular and molecular medicine 2019-12, Vol.23 (12), p.8019-8024
• Main Authors: Jiang, Liuqin, Ye, Bixing, Wang, Yun, Yu, Ting, Xu, Hairong
• Format: Article
• Language: English
• Subjects: 1-Phosphatidylinositol 3-kinase; Acetic acid; Acids; Agonists; AKT protein; Animals; Ca2; Calcium; Calcium (extracellular); Calcium (intracellular); Calcium - metabolism; Calcium ions; Capsaicin receptors; Colon - cytology; Colon - drug effects; Colon - metabolism; Fecal incontinence; Fluidity; Ganglia, Spinal - metabolism; Gene expression; Growth factors; Hypersensitivity; Intracellular; Irritable bowel syndrome; Kinases; Laboratory animals; Male; MAP kinase; MAP Kinase Signaling System - genetics; Metabolic pathways; mRNA; Nerve growth factor; Nerve Growth Factor - agonists; Nerve Growth Factor - antagonists & inhibitors; Nerve Growth Factor - genetics; Nerve Growth Factor - metabolism; Nervous system; NF-kappa B - metabolism; NGF‐mediated visceral sensitivity; Organs; Original; Phosphatidylinositol 3-Kinases - genetics; Phosphatidylinositol 3-Kinases - metabolism; Phosphoinositide-3 Kinase Inhibitors - pharmacology; Phospholipase C gamma - antagonists & inhibitors; Phospholipase C gamma - metabolism; protein TRPV1; Proteins; Proto-Oncogene Proteins c-akt - genetics; Proto-Oncogene Proteins c-akt - metabolism; pTRPV1; Rats; Receptor, trkA - genetics; Receptor, trkA - metabolism; Rodents; Sacrum; Sacrum - innervation; Signal transduction; Signal Transduction - drug effects; Signal Transduction - genetics; SNS; Stimulation; TrkA protein; TRPV Cation Channels - metabolism
• ISSN: 1582-1838; 1582-4934
• DOI: 10.1111/jcmm.14660

To investigate the efficacy of sacral nerve stimulation (SNS) on nerve growth factor (NGF) mediated visceral sensitivity in normal rat and visceral hypersensitivity model rats. 120 male newborn rats were randomly divided into 6 groups: group A was normal model group; group B ~ F were all sensitized with acetic acid enema and grouped again. Group c2 was given NGF antagonist, d2 group was given NGF agonist, e2 group was given PI3K inhibitor, and f2 group was given PLC‐γ inhibitor. After treatment, the expression of NGF, TrKA, PI3K, AKT, PLC‐γ, NF‐κB, TRPV1, pTRPV1 and intracellular Ca2+ content were detected. The expression of protein TRPV1 and pTRPV1 was increased, and Ca2+ was increased in the visceral hypersensitive group. NGF, TrKA in NGF antagonist group, PI3K, AKT, NF‐κB in PI3K inhibitor group, PLC‐γ in PLC‐γ inhibitor group were all almost not expressed. The relative expression of NGF, TrKA, PI3K, AKT, PLC‐γ and NF‐κB in NGF antagonist group was lower than that in visceral hypersensitivity group and NGF activator group (P