Reactive oxygen species (ROS) generation is stimulated by κ opioid receptor activation through phosphorylated c-Jun N-terminal kinase and inhibited by p38 mitogen-activated protein kinase (MAPK) activation

• Published in: The Journal of biological chemistry 2019-11, Vol.294 (45), p.16884-16896
• Main Authors: Schattauer, Selena S., Bedini, Andrea, Summers, Floyd, Reilly-Treat, Aiden, Andrews, Mackenzie M., Land, Benjamin B., Chavkin, Charles
• Format: Article
• Language: English
• Subjects: c-Jun N-terminal kinase (JNK); cell signaling; Enzyme Activation - drug effects; G protein; G protein-coupled receptor (GPCR); HEK293 Cells; Humans; JNK Mitogen-Activated Protein Kinases - metabolism; MAPK; opiate opioid; opioid; p38; p38 Mitogen-Activated Protein Kinases - metabolism; peroxiredoxin; Phosphorylation - drug effects; reactive oxygen; Reactive Oxygen Species - metabolism; Receptors, Opioid, kappa - agonists; Receptors, Opioid, kappa - metabolism; Signal Transduction
• ISSN: 0021-9258; 1083-351X; 1083-351X
• DOI: 10.1074/jbc.RA119.009592

Activation of the mitogen-activated protein kinase (MAPK) c-Jun N-terminal kinase (JNK) by the Gi/o protein–coupled κ opioid receptor (KOR), μ opioid, and D2 dopamine receptors stimulates peroxiredoxin 6 (PRDX6)-mediated production of reactive oxygen species (ROS). ROS production by KOR-inactivating antagonists norbinaltorphimine (norBNI) and JDTic blocks Gαi protein activation, but the signaling mechanisms and consequences of JNK activation by KOR agonists remain uncharacterized. Binding of arrestins to KOR causes desensitization of G protein signaling and acts as a scaffold to initiate MAPK activation. Here, we found that the KOR agonists U50,488 and dynorphin B stimulated biphasic JNK activation with an early arrestin-independent phase, requiring the small G protein RAC family small GTPase 1 (RAC1) and protein kinase C (PKC), and a later arrestin-scaffolded phase, requiring RAC1 and Ras homolog family member (RHO) kinase. JNK activation by U50,488 and dynorphin B also stimulated PRDX6-dependent ROS production but with an inverted U-shaped dose-response relationship. KOR agonist-induced ROS generation resulted from the early arrestin-independent phase of JNK activation, and this ROS response was suppressed by arrestin-dependent activation of the MAPK p38. The apparent balance between p38 MAPK and JNK/ROS signaling has important physiological implications for understanding of dynorphin activities during the stress response. To visualize these activities, we monitored KOR agonist–mediated activation of ROS in transfected live cells by two fluorescent sensors, CellROX Green and HyPerRed. These findings establish an important aspect of opioid receptor signaling and suggest that ROS induction may be part of the physiological response to KOR activation.