Melatonin and Caffeine Supplementation Used, Respectively, as Protective and Stimulating Agents in the Cryopreservation of Human Sperm Improves Survival, Viability, and Motility after Thawing compared to Traditional TEST-Yolk Buffer

Cryopreservation processes can damage spermatozoa and impair structural and functional cell characteristics. Plasma, nuclear membranes, and cellular organelles can suffer from the freeze and thaw process. This study evaluates the protective and stimulant effect of melatonin and caffeine supplementat...

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Published in:Oxidative medicine and cellular longevity 2019, Vol.2019 (2019), p.1-10
Main Authors: Drevet, J. R., Evenson, Donald P., Monteiro, Rosa A. C., Ranéa, Caroline, Pariz, Juliana R., Hallak, Jorge
Format: Article
Language:English
Subjects:
Adult
Artificial insemination
Buffers
Caffeine
Caffeine - pharmacology
Cell Survival - drug effects
Comparative analysis
Cryopreservation
Ethylenediaminetetraacetic acid
Humans
Male
Melatonin
Melatonin - pharmacology
Microsurgery
Middle Aged
Motility
Oxidative stress
Reactive oxygen species
Reactive Oxygen Species - metabolism
Sperm
Sperm Motility - drug effects
Spermatozoa
Spermatozoa - cytology
Spermatozoa - metabolism
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description Cryopreservation processes can damage spermatozoa and impair structural and functional cell characteristics. Plasma, nuclear membranes, and cellular organelles can suffer from the freeze and thaw process. This study evaluates the protective and stimulant effect of melatonin and caffeine supplementation on the functional characteristics of human spermatozoa before and after freezing. Thirty seminal samples from normozoospermic men aged 19–45 years old collected between October 2012 and May 2017 were included. Semen samples were supplemented with either 2 mM melatonin (MEL) prior to cryopreservation, 2 mM caffeine (CAF) in postthaw, or CAF and MEL (CM) in precryopreservation and postthaw, respectively. Kinetics and seminal parameters, mitochondrial activity, DNA fragmentation, and reactive oxygen species (ROS) levels were analyzed before and after cryopreservation. A significant reduction in sperm concentration, total and progressive motility, sperm kinetics, and mitochondrial activity, as well as a significant increase in DNA fragmentation and ROS production in postthaw samples compared to fresh samples, was identified. After administration of a caffeine and/or melatonin supplement, there was a significant increase in progressive motility in the CAF (p=0.005) and CM (p=0.048) groups, as well as mitochondrial activity in the CM group (p
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R. ; Evenson, Donald P. ; Monteiro, Rosa A. C. ; Ranéa, Caroline ; Pariz, Juliana R. ; Hallak, Jorge</creator><contributor>Georgakilas, Alexandros ; Alexandros Georgakilas</contributor><creatorcontrib>Drevet, J. R. ; Evenson, Donald P. ; Monteiro, Rosa A. C. ; Ranéa, Caroline ; Pariz, Juliana R. ; Hallak, Jorge ; Georgakilas, Alexandros ; Alexandros Georgakilas</creatorcontrib><description>Cryopreservation processes can damage spermatozoa and impair structural and functional cell characteristics. Plasma, nuclear membranes, and cellular organelles can suffer from the freeze and thaw process. This study evaluates the protective and stimulant effect of melatonin and caffeine supplementation on the functional characteristics of human spermatozoa before and after freezing. Thirty seminal samples from normozoospermic men aged 19–45 years old collected between October 2012 and May 2017 were included. Semen samples were supplemented with either 2 mM melatonin (MEL) prior to cryopreservation, 2 mM caffeine (CAF) in postthaw, or CAF and MEL (CM) in precryopreservation and postthaw, respectively. Kinetics and seminal parameters, mitochondrial activity, DNA fragmentation, and reactive oxygen species (ROS) levels were analyzed before and after cryopreservation. A significant reduction in sperm concentration, total and progressive motility, sperm kinetics, and mitochondrial activity, as well as a significant increase in DNA fragmentation and ROS production in postthaw samples compared to fresh samples, was identified. After administration of a caffeine and/or melatonin supplement, there was a significant increase in progressive motility in the CAF (p=0.005) and CM (p=0.048) groups, as well as mitochondrial activity in the CM group (p&lt;0.05). Cryopreservation has negative effects on overall sperm quality and increases ROS production. 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R.</au><au>Evenson, Donald P.</au><au>Monteiro, Rosa A. C.</au><au>Ranéa, Caroline</au><au>Pariz, Juliana R.</au><au>Hallak, Jorge</au><au>Georgakilas, Alexandros</au><au>Alexandros Georgakilas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Melatonin and Caffeine Supplementation Used, Respectively, as Protective and Stimulating Agents in the Cryopreservation of Human Sperm Improves Survival, Viability, and Motility after Thawing compared to Traditional TEST-Yolk Buffer</atitle><jtitle>Oxidative medicine and cellular longevity</jtitle><addtitle>Oxid Med Cell Longev</addtitle><date>2019</date><risdate>2019</risdate><volume>2019</volume><issue>2019</issue><spage>1</spage><epage>10</epage><pages>1-10</pages><issn>1942-0900</issn><eissn>1942-0994</eissn><abstract>Cryopreservation processes can damage spermatozoa and impair structural and functional cell characteristics. 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A significant reduction in sperm concentration, total and progressive motility, sperm kinetics, and mitochondrial activity, as well as a significant increase in DNA fragmentation and ROS production in postthaw samples compared to fresh samples, was identified. After administration of a caffeine and/or melatonin supplement, there was a significant increase in progressive motility in the CAF (p=0.005) and CM (p=0.048) groups, as well as mitochondrial activity in the CM group (p&lt;0.05). Cryopreservation has negative effects on overall sperm quality and increases ROS production. A combination of caffeine and melatonin in prefreeze and postthaw sperm samples has proven to be a very effective and simple way to improve semen quality. 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subjects Adult
Artificial insemination
Buffers
Caffeine
Caffeine - pharmacology
Cell Survival - drug effects
Comparative analysis
Cryopreservation
Ethylenediaminetetraacetic acid
Humans
Male
Melatonin
Melatonin - pharmacology
Microsurgery
Middle Aged
Motility
Oxidative stress
Reactive oxygen species
Reactive Oxygen Species - metabolism
Sperm
Sperm Motility - drug effects
Spermatozoa
Spermatozoa - cytology
Spermatozoa - metabolism
title Melatonin and Caffeine Supplementation Used, Respectively, as Protective and Stimulating Agents in the Cryopreservation of Human Sperm Improves Survival, Viability, and Motility after Thawing compared to Traditional TEST-Yolk Buffer
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