The Development of Functional Non-Viral Vectors for Gene Delivery

Gene therapy is manipulation in/of gene expression in specific cells/tissue to treat diseases. This manipulation is carried out by introducing exogenous nucleic acids, such as DNA or RNA, into the cell. Because of their negative charge and considerable larger size, the delivery of these molecules, i...

Full description

Saved in:
Bibliographic Details
Published in:International journal of molecular sciences 2019-11, Vol.20 (21), p.5491
Main Authors: Patil, Suryaji, Gao, Yong-Guang, Lin, Xiao, Li, Yu, Dang, Kai, Tian, Ye, Zhang, Wen-Juan, Jiang, Shan-Feng, Qadir, Abdul, Qian, Ai-Rong
Format: Article
Language:English
Subjects:
Acids
Animals
Biodegradation
Cationic polymerization
Cystic fibrosis
Cytotoxicity
Deoxyribonucleic acid
DNA
Efficiency
Expression vectors
Fluorescence
Gene expression
Gene therapy
Gene transfer
Gene Transfer Techniques
Genetic Therapy - methods
Genetic Vectors - adverse effects
Genetic Vectors - genetics
Humans
Immunogenicity
Lipids
Molecular weight
Nanoparticles
Nanoparticles - chemistry
Nanoparticles - metabolism
Nucleic acids
Polymers
Quantum dots
Review
Transfection
Vectors (Biology)
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Gene therapy is manipulation in/of gene expression in specific cells/tissue to treat diseases. This manipulation is carried out by introducing exogenous nucleic acids, such as DNA or RNA, into the cell. Because of their negative charge and considerable larger size, the delivery of these molecules, in general, should be mediated by gene vectors. Non-viral vectors, as promising delivery systems, have received considerable attention due to their low cytotoxicity and non-immunogenicity. As research continued, more and more functional non-viral vectors have emerged. They not only have the ability to deliver a gene into the cells but also have other functions, such as the performance of fluorescence imaging, which aids in monitoring their progress, targeted delivery, and biodegradation. Recently, many reviews related to non-viral vectors, such as polymers and cationic lipids, have been reported. However, there are few reviews regarding functional non-viral vectors. This review summarizes the common functional non-viral vectors developed in the last ten years and their potential applications in the future. The transfection efficiency and the transport mechanism of these materials were also discussed in detail. We hope that this review can help researchers design more new high-efficiency and low-toxicity multifunctional non-viral vectors, and further accelerate the progress of gene therapy.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms20215491