Effect of stressful conditions on the carotenogenic activity of a Colombian strain of Dunaliella salina

• Published in: Saudi journal of biological sciences 2019-11, Vol.26 (7), p.1325-1330
• Main Authors: Gallego-Cartagena, Euler, Castillo-Ramírez, Margarita, Martínez-Burgos, Walter
• Format: Article
• Language: English
• Subjects: Biotechnological purposes; Carotenoides; Dunaliella salina; Stressful conditions; الآثار الفسيولوجية; المسرطنات; دوناليلا سالينا
• ISSN: 1319-562X; 2213-7106
• DOI: 10.1016/j.sjbs.2019.07.010

The objective was evaluate the carotenogenic activity of Dunaliella salina isolated from the artificial salt flats of municipality of Manaure (Department of La Guajira, Colombia). Two experimental testings were designed, in triplicate, to induce the reversibility of the cell tonality depending on the culture conditions. In the first test (A), to induce the reversibility from green to red tonality in D. salina cells, these were cultured in J/1 medium at a concentration of 4.0 M NaCl, 390 µmol m−2 s−1, 0.50 mM KNO3. In the second test (B), to induce the reversibility from red to green cell tonality, the cultures were maintained in J/1 medium 1 M NaCl, 190 µmol m−2 s−1, 5.0 mM KNO3 and pH 8.2. The population growth was evaluated by cell count and the pigment content was performed by spectrophotometric techniques. It was found that in both tests the culture conditions influenced the population growth and the pigments production of D. salina. There was a significant difference between the mean values of total carotenoids in the test A with 9.67 ± 0.19 μg/ml and second test with 1.54 ± 0.08 μg/ml at a significance level of p