Airway epithelial regeneration requires autophagy and glucose metabolism

• Published in: Cell death & disease 2019-11, Vol.10 (12), p.875-14, Article 875
• Main Authors: Li, Kuan, Li, Minmin, Li, Wenli, Yu, Hongzhi, Sun, Xin, Zhang, Qiuyang, Li, Yu, Li, Xue, Li, Yue, Abel, E. Dale, Wu, Qi, Chen, Huaiyong
• Format: Article
• Language: English
• Subjects: 13/106; 13/31; 14/63; 38/77; 631/136/532/1360; 631/532/489; 64/60; Animals; Antibodies; Autophagy; Biochemistry; Biomedical and Life Sciences; Cell Biology; Cell Culture; Cell differentiation; Cell Differentiation - physiology; Cell proliferation; Epithelial Cells - cytology; Epithelial Cells - metabolism; Epithelial Cells - physiology; Epithelium; Glucose; Glucose - metabolism; Glucose transporter; Glycolysis; Humans; Hypersensitivity; Immunology; Inflammation; Life Sciences; Lung - cytology; Lung - metabolism; Lung - physiology; Lungs; Metabolism; Mice; Mice, Inbred C57BL; Organoids; Ovalbumin; Phagocytosis; Progenitor cells; Regeneration - physiology; Respiratory tract; Stem cells; Stem Cells - cytology; Stem Cells - metabolism; Stem Cells - physiology
• ISSN: 2041-4889; 2041-4889
• DOI: 10.1038/s41419-019-2111-2

Efficient repair of injured epithelium by airway progenitor cells could prevent acute inflammation from progressing into chronic phase in lung. Here, we used small molecules, genetic loss-of-function, organoid cultures, and in vivo lung-injury models to show that autophagy is essential for maintaining the pool of airway stem-like vClub cells by promoting their proliferation during ovalbumin-induced acute inflammation. Mechanistically, impaired autophagy disrupted glucose uptake in vClub progenitor cells, and either reduced accessibility to glucose or partial inhibition of glycolysis promoted the proliferative capacity of vClub progenitor cells and their daughter Club cells. However, glucose deprivation or glycolysis blockade abrogated the proliferative capacity of airway vClub cells and Club cells but promoted ciliated and goblet cell differentiation. Deficiency of glucose transporter-1 suppressed the proliferative capacity of airway progenitor cells after ovalbumin challenge. These findings suggested that autophagy and glucose metabolism are essential for the maintenance of airway epithelium at steady state and during allergic inflammation.