R-Loops Promote Antisense Transcription across the Mammalian Genome

• Published in: Molecular cell 2019-11, Vol.76 (4), p.600-616.e6
• Main Authors: Tan-Wong, Sue Mei, Dhir, Somdutta, Proudfoot, Nick J.
• Format: Article
• Language: English
• Subjects: antisense lncRNA; enhancer RNA; Genome, Human; HEK293 Cells; HeLa Cells; human HeLa cells; Humans; promoter activity; Promoter Regions, Genetic; R-loop; R-Loop Structures; Ribonuclease H - metabolism; RNA polymerase II; RNA, Antisense - biosynthesis; RNA, Antisense - genetics; RNA, Long Noncoding - biosynthesis; RNA, Long Noncoding - genetics; RNase H1; single-stranded DNA; Structure-Activity Relationship; Transcription, Genetic; Transcriptional Activation
• ISSN: 1097-2765; 1097-4164
• DOI: 10.1016/j.molcel.2019.10.002

Widespread antisense long noncoding RNA (lncRNA) overlap with many protein-coding genes in mammals and emanate from gene promoter, enhancer, and termination regions. However, their origin and biological purpose remain unclear. We show that these antisense lncRNA can be generated by R-loops that form when nascent transcript invades the DNA duplex behind elongating RNA polymerase II (Pol II). Biochemically, R-loops act as intrinsic Pol II promoters to induce de novo RNA synthesis. Furthermore, their removal across the human genome by RNase H1 overexpression causes the selective reduction of antisense transcription. Consequently, we predict that R-loops act to facilitate the synthesis of many gene proximal antisense lncRNA. Not only are R-loops widely associated with DNA damage and repair, but we now show that they have the capacity to promote de novo transcript synthesis that may have aided the evolution of gene regulation. [Display omitted] •R-loops formed within plasmids promote antisense transcription in nuclear extracts•TSS of lncRNA and eRNA are often near R-loop structures and sensitive to RNase H1•Preinitiation complexes associated with lncRNA synthesis are R-loop dependent•Many mammalian lncRNA derive from R-loop promoter activity Tan-Wong et al. demonstrate that R-loop structures, often formed at the promoter, terminator, and enhancer regions of human protein-coding genes, act as promoters to generate antisense lncRNA. In effect, R-loop promoter activity may account for the existence of many lncRNA that are detected across mammalian genomes.