Untargeted histone profiling during naive conversion uncovers conserved modification markers between mouse and human

Recent progress has enabled the conversion of primed human embryonic stem cells (hESCs) to the naive state of pluripotency, resembling the well-characterized naive mouse ESCs (mESCs). However, a thorough histone epigenetic characterization of this conversion process is currently lacking, while its l...

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Published in:Scientific reports 2019-11, Vol.9 (1), p.17240-11, Article 17240
Main Authors: De Clerck, Laura, Taelman, Jasin, Popovic, Mina, Willems, Sander, Van der Jeught, Margot, Heindryckx, Björn, De Sutter, Petra, Marks, Hendrik, Deforce, Dieter, Dhaenens, Maarten
Format: Article
Language:English
Subjects:
13/1
38/77
631/208/176
631/532/2064/2117
631/61/475
631/80/458
82/16
82/58
96/100
Animals
Biomarkers - metabolism
Cell Differentiation - physiology
Cells, Cultured
Embryo cells
Embryos
Epigenetics
Epigenome - physiology
Experimental design
Histones - metabolism
Human Embryonic Stem Cells - metabolism
Humanities and Social Sciences
Humans
Mass spectrometry
Mass spectroscopy
Mice
multidisciplinary
Pluripotency
Pluripotent Stem Cells - metabolism
Post-translation
Science
Science (multidisciplinary)
Signal Transduction - physiology
Stem cell transplantation
Stem cells
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Deforce, Dieter
Dhaenens, Maarten
description Recent progress has enabled the conversion of primed human embryonic stem cells (hESCs) to the naive state of pluripotency, resembling the well-characterized naive mouse ESCs (mESCs). However, a thorough histone epigenetic characterization of this conversion process is currently lacking, while its likeness to the mouse model has not been clearly established. Here, we profile the histone epigenome of hESCs during conversion in a time-resolved experimental design, using an untargeted mass spectrometry-based approach. In total, 23 histone post-translational modifications (hPTMs) changed significantly over time. H3K27Me3 was the most prominently increasing marker hPTM in naive hESCs. This is in line with previous reports in mouse, prompting us to compare all the shared hPTM fold changes between mouse and human, revealing a set of conserved hPTM markers for the naive state. Principally, we present the first roadmap of the changing human histone epigenome during the conversion of hESCs from the primed to the naive state. This further revealed similarities with mouse, which hint at a conserved mammalian epigenetic signature of the ground state of pluripotency.
doi_str_mv 10.1038/s41598-019-53681-6
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subjects 13/1
38/77
631/208/176
631/532/2064/2117
631/61/475
631/80/458
82/16
82/58
96/100
Animals
Biomarkers - metabolism
Cell Differentiation - physiology
Cells, Cultured
Embryo cells
Embryos
Epigenetics
Epigenome - physiology
Experimental design
Histones - metabolism
Human Embryonic Stem Cells - metabolism
Humanities and Social Sciences
Humans
Mass spectrometry
Mass spectroscopy
Mice
multidisciplinary
Pluripotency
Pluripotent Stem Cells - metabolism
Post-translation
Science
Science (multidisciplinary)
Signal Transduction - physiology
Stem cell transplantation
Stem cells
title Untargeted histone profiling during naive conversion uncovers conserved modification markers between mouse and human
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