Innovative particle standards and long-lived imaging for 2D and 3D dSTORM

Direct stochastic optical reconstruction microscopy (dSTORM), developed in the last decade, has revolutionised optical microscopy by enabling scientists to visualise objects beyond the resolution provided by conventional microscopy (200 nm). We developed an innovative method based on blinking partic...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2019-11, Vol.9 (1), p.17967-13, Article 17967
Main Authors: Provost, Angelina, Rousset, Corentin, Bourdon, Laura, Mezhoud, Sarra, Reungoat, Emma, Fourneaux, Camille, Bresson, Timothée, Pauly, Marine, Béard, Nicolas, Possi-Tchouanlong, Laura, Grigorov, Boyan, Bouvet, Philippe, Diaz, Jean-Jacques, Chamot, Christophe, Pécheur, Eve-Isabelle, Ladavière, Catherine, Charreyre, Marie-Thérèse, Favier, Arnaud, Place, Christophe, Monier, Karine
Format: Article
Language:English
Subjects:
14
14/34
14/63
631/1647/245
631/337/2265
631/80/128/1965
639/638/439/945
639/925/350/2251
Biochemistry, Molecular Biology
Biophysics
Centrosomes
Humanities and Social Sciences
Life Sciences
Light microscopy
Microscopy
multidisciplinary
Science
Science (multidisciplinary)
Virions
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Direct stochastic optical reconstruction microscopy (dSTORM), developed in the last decade, has revolutionised optical microscopy by enabling scientists to visualise objects beyond the resolution provided by conventional microscopy (200 nm). We developed an innovative method based on blinking particle standards and conditions for long-lived imaging over several weeks. Stable localisation precisions within the 10 nm-range were achieved for single virions and in cellulo 2D imaging of centrosomes, as well as their reliable reconstruction in 3D dSTORM.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-019-53528-0