Overexpression of Inducible Nitric Oxide Synthase in Allergic and Nonallergic Nasal Polyp

Sinonasal polyps are very common benign lesions of the nasal mucosa. Most of nasal polyps (NP) are idiopathic, and the pathophysiology of this disease is still incompletely understood. Nitric oxide (NO) is a reactive molecule generated by nitric oxide synthase (NOS). NO has been identified as an imp...

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Bibliographic Details
Published in:Oxidative medicine and cellular longevity 2019, Vol.2019 (2019), p.1-11
Main Authors: Althubiti, Mohammad Ahmad, El-Readi, Mahmoud Zaki, Eid, Safaa Yehia, Almaimani, Riyad A., Sadek, Ahmed Adel, Abdelwahab, Soha
Format: Article
Language:English
Subjects:
Adolescent
Adult
Allergies
Archives & records
Aspirin
Asthma
Biomarkers - metabolism
Case-Control Studies
Female
Gene expression
Humans
Hypersensitivity - diagnosis
Hypersensitivity - genetics
Hypersensitivity - metabolism
Immunology
Inflammation - diagnosis
Inflammation - genetics
Inflammation - metabolism
Male
Middle Aged
Nasal Polyps - diagnosis
Nasal Polyps - genetics
Nasal Polyps - metabolism
Nitric oxide
Nitric Oxide - metabolism
Nitric Oxide Synthase Type II - genetics
Nitric Oxide Synthase Type II - metabolism
Nose
Otolaryngology
Otology
Pathogenesis
Patients
Polyps
Prospective Studies
Sinuses
Surgery
Young Adult
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Summary:Sinonasal polyps are very common benign lesions of the nasal mucosa. Most of nasal polyps (NP) are idiopathic, and the pathophysiology of this disease is still incompletely understood. Nitric oxide (NO) is a reactive molecule generated by nitric oxide synthase (NOS). NO has been identified as an important mediator in airway function and pathogenesis of several respiratory system diseases. Histological and genetical expression of iNOS was detected to evaluate the role of NO in the pathogenesis of allergic (ANP) and nonallergic nasal polyps (NANP). Forty patients with nasal polyps (20 allergic and 20 nonallergic) were identified by history, clinical examination, and investigation. NPs were obtained from the middle turbinate (MT) during concha bullosa surgery. Twenty normal MT nasal tissues were taken as the control from patients undergoing concha bullosa surgery, without any evidence of allergy or inflammation. A nasal polyp specimen from each patient was subjected for immune-histochemical study followed by histological examination to detect the expression of iNOS. RT-PCR was used to evaluate the iNOS gene expression in isolated tissues. The expression of iNOS in both epithelial and stromal layers was greater in NP than in MT tissues. The ANP group showed more iNOS expression than those of the NANP group. The relative mRNA levels of iNOS gene were significantly higher in ANP (2.5-fold) compared to the normal (1.02-fold, P
ISSN:1942-0900
1942-0994
DOI:10.1155/2019/7506103