Molecular phenotyping and image-guided surgical treatment of melanoma using spectrally distinct ultrasmall core-shell silica nanoparticles

Accurate detection and quantification of metastases in regional lymph nodes remain a vital prognostic predictor for cancer staging and clinical outcomes. As intratumoral heterogeneity poses a major hurdle to effective treatment planning, more reliable image-guided, cancer-targeted optical multiplexi...

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Bibliographic Details
Published in:Science advances 2019-12, Vol.5 (12), p.eaax5208
Main Authors: Chen, Feng, Madajewski, Brian, Ma, Kai, Karassawa Zanoni, Daniella, Stambuk, Hilda, Turker, Melik Z, Monette, Sébastien, Zhang, Li, Yoo, Barney, Chen, Peiming, Meester, Richard J C, de Jonge, Sander, Montero, Pablo, Phillips, Evan, Quinn, Thomas P, Gönen, Mithat, Sequeira, Sonia, de Stanchina, Elisa, Zanzonico, Pat, Wiesner, Ulrich, Patel, Snehal G, Bradbury, Michelle S
Format: Article
Language:English
Subjects:
Animals
Cell Line, Tumor
Health and Medicine
Lymph Nodes - diagnostic imaging
Lymph Nodes - pathology
Lymphatic Metastasis - pathology
Melanoma - diagnostic imaging
Melanoma - genetics
Melanoma - surgery
Mice
Multimodal Imaging
Nanoparticles - chemistry
Nanoparticles - ultrastructure
Optical Imaging
Particle Size
Phenotype
Positron Emission Tomography Computed Tomography
SciAdv r-articles
Sentinel Lymph Node Biopsy
Silicon Dioxide - chemistry
Surgery, Computer-Assisted
Swine
Swine, Miniature
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Summary:Accurate detection and quantification of metastases in regional lymph nodes remain a vital prognostic predictor for cancer staging and clinical outcomes. As intratumoral heterogeneity poses a major hurdle to effective treatment planning, more reliable image-guided, cancer-targeted optical multiplexing tools are critically needed in the operative suite. For sentinel lymph node mapping indications, accurately interrogating distinct molecular signatures on cancer cells in vivo with differential levels of sensitivity and specificity remains largely unexplored. To address these challenges and demonstrate sensitivity to detecting micrometastases, we developed batches of spectrally distinct 6-nm near-infrared fluorescent core-shell silica nanoparticles, each batch surface-functionalized with different melanoma targeting ligands. Along with PET imaging, particles accurately detected and molecularly phenotyped cancerous nodes in a spontaneous melanoma miniswine model using image-guided multiplexing tools. Information afforded from these tools offers the potential to not only improve the accuracy of targeted disease removal and patient safety, but to transform surgical decision-making for oncological patients.
ISSN:2375-2548
2375-2548
DOI:10.1126/sciadv.aax5208