CD105 (Endoglin) as negative prognostic factor in AML

• Published in: Scientific reports 2019-12, Vol.9 (1), p.18337-11, Article 18337
• Main Authors: Kauer, Joseph, Schwartz, Karolin, Tandler, Claudia, Hinterleitner, Clemens, Roerden, Malte, Jung, Gundram, Salih, Helmut R., Heitmann, Jonas S., Märklin, Melanie
• Format: Article
• Language: English
• Subjects: 13/1; 13/51; 42/109; 631/250; 692/4028/67/1990/283/1897; Acute myeloid leukemia; Adult; Aged; Aged, 80 and over; Biomarkers, Tumor - blood; CD105 antigen; Cell proliferation; Disease-Free Survival; Endoglin; Endoglin - blood; Endoglin - genetics; Endothelial cells; Endothelial Cells - metabolism; Endothelial Cells - pathology; Fab; Female; Flow cytometry; Gene Expression Regulation, Leukemic - genetics; Hematology; Humanities and Social Sciences; Humans; Leukemia; Leukemia, Myeloid, Acute - blood; Leukemia, Myeloid, Acute - genetics; Leukemia, Myeloid, Acute - pathology; Male; Middle Aged; multidisciplinary; Neovascularization, Pathologic - blood; Neovascularization, Pathologic - pathology; Prognosis; Science; Science (multidisciplinary); Solid tumors
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-019-54767-x

While several genetic and morphological markers are established and serve to guide therapy of acute myeloid leukaemia (AML), there is still profound need to identify additional markers to better stratify patients. CD105 (Endoglin) is a type I transmembrane protein reported to induce activation and proliferation of endothelial cells. In addition, CD105 is expressed in haematological malignancies and the vessels of solid tumours. Here, CD105 associates with unfavourable disease course, but so far no data are available on the prognostic relevance of CD105 in haematological malignancies. We here generated a novel CD105 antibody for analysis of expression and prognostic relevance of CD105 in a cohort of 62 AML patients. Flow cytometric analysis revealed substantial expression in the various AML FAB types, with FAB M3 type displaying significantly lower surface levels. Next we established a cut-off specific fluorescence level of 5.22 using receiver-operating characteristics, which allowed to group patients in cases with CD105 lo and CD105 hi surface expression and revealed that high CD105 expression correlated significantly with poor overall and progression free survival. In conclusion, we here identify CD105 expression as a novel prognostic marker in AML, which may serve to optimize follow up and treatment decisions for AML patients.