Black Ginseng and Ginsenoside Rb1 Promote Browning by Inducing UCP1 Expression in 3T3-L1 and Primary White Adipocytes

• Published in: Nutrients 2019-11, Vol.11 (11), p.2747
• Main Authors: Park, Seon-Joo, Park, Miey, Sharma, Anshul, Kim, Kihyun, Lee, Hae-Jeung
• Format: Article
• Language: English
• Subjects: 3T3-L1 Cells; Adipocytes; Adipocytes, Brown - drug effects; Adipocytes, Brown - metabolism; Adipocytes, White - drug effects; Adipocytes, White - metabolism; Adipogenesis - drug effects; AMP-Activated Protein Kinases - metabolism; Animals; Anti-Obesity Agents - pharmacology; Antibiotics; Browning; CCAAT-Enhancer-Binding Proteins - metabolism; Dietary restrictions; DNA-Binding Proteins - metabolism; Energy; Gene expression; Ginseng; Ginsenosides - pharmacology; Homeostasis; Kinases; Laboratory animals; Male; Metabolic disorders; Metabolism; Mice; Mice, Inbred C57BL; Mitochondria - drug effects; Mitochondria - metabolism; Nutrition research; Obesity; Organelle Biogenesis; Panax - chemistry; Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha - metabolism; Physical activity; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; PPAR gamma - metabolism; Proteins; Risk analysis; Risk factors; Signal Transduction; Sterol Regulatory Element Binding Protein 1 - metabolism; Thermogenesis; Transcription Factors - metabolism; Uncoupling Protein 1 - metabolism; Up-Regulation
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu11112747

In this study, we investigated the effects of black ginseng (BG) and ginsenoside Rb1, which induced browning effects in 3T3-L1 and primary white adipocytes (PWATs) isolated from C57BL/6 mice. BG and Rb1 suppressed the expressions of CCAAT/enhancer-binding protein alpha (C/EBPα) and sterol regulatory element-binding transcription factor-1c (SREBP-1c), whereas the expression level of peroxisome proliferator-activated receptor gamma (PPARγ) was increased. Furthermore, BG and Rb1 enhanced the protein expressions of the brown-adipocyte-specific markers PR domain containing 16 (PRDM16), peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1α), and uncoupling protein 1 (UCP1). These results were further supported by immunofluorescence images of mitochondrial biogenesis. In addition, BG and Rb1 induced expressions of brown-adipocyte-specific marker proteins by AMP-activated protein kinase (AMPK) activation. BG and Rb1 exert antiobesity effects by inducing browning in 3T3-L1 cells and PWATs through AMPK-mediated pathway activation. We suggest that BG and Rb1 act as potential functional antiobesity food agents.