286 Effects of dietary peroxidized lipids and antioxidant supplementation in the drinking water on growth, oxidative and immune status of newly weaned piglets

• Published in: Journal of animal science 2019-12, Vol.97 (Supplement_3), p.119-119
• Main Authors: Silva-Guillen, Ysenia Victoria, Martinez, Gabriela, Arellano, Consuelo, Heugten, Eric van
• Format: Article
• Language: English
• Subjects: ORAL PRESENTATIONS
• ISSN: 0021-8812; 1525-3163
• DOI: 10.1093/jas/skz258.246

The objective of the present study was to evaluate the use of vitamin E (VitE) and phytogenic compounds (PHYTO) in drinking water on performance, oxidative stress, and immune status of weaned piglets fed peroxidized lipids. In a 35-day study, 21-d old weaned piglets (n = 96; 6.10±0.64 kg BW) were assigned within sex and BW blocks to 1 of 4 dietary treatments, using 24 pens (4 pigs/pen; 6 replications/treatment). Diets contained either 6% human-grade soybean oil or 6% soybean oil which was peroxidized for 12 d at 80°C with constant air flow at 50 L/min (initial peroxide value, anisidine value, hexanal, and 2,4-decadienal concentrations were 4.1 vs. 141.6 meq/kg, 1.7 vs. 106, < 5 vs. 99 mg/kg, and 8 vs. 720 mg/kg for control and peroxidized oil, respectively). Pigs fed peroxidized lipids received drinking water without (control) or with supplemental VitE (100 IU/L d-α-tocopherol) or PHYTO (60 µL/L from d 1–7; 30 µL/L from d 8–35). Pigs fed control diet received control water only. Overall, peroxidized lipids decreased (P < 0.001) BW (18.20 vs. 21.55 kg) and ADG (347 vs. 441 g/day), and tended to decrease ADFI (P = 0.14; 537 vs. 617 g/day) and G:F (P = 0.07; 645 vs. 715 g/kg). Peroxidation decreased serum vitamin E (P = 0.03) which could be restored (P = 0.01) by VitE in the water, but not PHYTO. Serum concentrations of interferon-γ, interleukin(IL)-1α, IL-1β, IL-1ra, IL2, IL4, IL-6, IL10, IL-12, IL-18, TNF-α, malondialdehyde, protein carbonyl, and total antioxidant capacity were not impacted by treatments (P > 0.05). Serum 8-hydroxydeoxyguanosine was reduced (P = 0.001) with feeding peroxidized lipids and this was not altered by supplemental antioxidants (P = 0.45). Peroxidized lipids clearly reduced growth performance, which did not appear to be related to oxidative stress markers or immune-regulatory cytokines. The negative effects of peroxidized lipids could not be improved by vitamin E or PHYTO supplemented in drinking water.