PSI-24 Poor maternal nutrition and gestational age affect oxidative stress in offspring muscle

• Published in: Journal of animal science 2019-12, Vol.97 (Supplement_3), p.251-251
• Main Authors: Iannitti, Helen R, Dias, Brittany A, Jones, Amanda K, Martin, Dominique E, Pillai, Sambhu, Hoffman, Maria L, Govoni, Kristen E, Zinn, Steven A, Reed, Sarah A
• Format: Article
• Language: English
• Subjects: POSTER PRESENTATIONS
• ISSN: 0021-8812; 1525-3163
• DOI: 10.1093/jas/skz258.510

Poor maternal nutrition during gestation decreases growth, increases lipid accumulation, and influences metabolism in offspring muscle. Oxidative stress is associated with lipid and protein damage and changes in metabolic function during fetal development which can persist postnatally. We hypothesized that poor maternal nutrition during gestation would increase oxidative stress in offspring muscle. To test this, 47 individually housed pregnant ewes received diets containing 60% (restricted-fed), 100% (control-fed), or 140% (over-fed) of National Research Council requirements for TDN beginning on day 30 ± 0.2 of gestation. Ewes were euthanized and offspring longissimus muscles (LM) were collected at d 90 or 135 of gestation, or within 24 h of birth (n = 8 to 14 offspring per maternal diet per gestational day). Offspring are referred to as RES, CON, and OVER, respectively. Glutathione, malondialdehyde, and protein carbonyl concentrations were determined in LM homogenates using commercial assays. Data were analyzed for effects of maternal diet, day of gestation, and their interaction using the glimmix procedure in SAS. Offspring born to over-fed ewes had reduced glutathione concentrations compared with offspring born to control-fed and restricted-fed ewes (OVER: 1.82 ± 0.30, CON: 5.58 ± 0.29, RES: 5.66 ± 0.29, M/g; P < 0.001). Malondialdehyde concentration increased in offspring LM from d 90 to d 135 and birth (d 90: 9.48 ± 2.2, d 135: 16.7 ± 2.1, birth: 20.9 ± 1.8 M/g; P < 0.001). Protein carbonylation was increased at d 90 compared with d 135 and birth (d 90: 8.05 ± 0.80, d 135: 5.68 ± 0.83, birth: 3.82 ± 0.84 g/mg; P = 0.003). Thus, gestational age influences lipid and protein oxidation. Poor maternal nutrition affects oxidative status in ovine offspring LM, which may negatively affect offspring muscle growth and development.