Pseudouridine synthase 7 impacts Candida albicans rRNA processing and morphological plasticity

RNA can be modified in over 100 distinct ways, and these modifications are critical for function. Pseudouridine synthases catalyse pseudouridylation, one of the most prevalent RNA modifications. Pseudouridine synthase 7 modifies a variety of substrates in Saccharomyces cerevisiae including tRNA, rRN...

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Bibliographic Details
Published in:Yeast (Chichester, England) England), 2019-11, Vol.36 (11), p.669-677
Main Authors: Pickerill, Ethan S., Kurtz, Rebecca P., Tharp, Aaron, Guerrero Sanz, Paula, Begum, Munni, Bernstein, Douglas A.
Format: Article
Language:English
Subjects:
Animals
Antifungal Agents - pharmacology
C. albicans
Candida albicans
Candida albicans - drug effects
Candida albicans - enzymology
Candida albicans - genetics
Candidiasis - microbiology
Cell surface
CRISPR
CRISPR-Cas Systems
Filamentation
Gene Deletion
Gene Editing
Genome editing
Genomes
Humans
Hydrophobic and Hydrophilic Interactions
Hydrophobicity
Intramolecular Transferases - genetics
Intramolecular Transferases - metabolism
Larva - microbiology
Moths - microbiology
mRNA
pseudouridine
Pseudouridine synthase
pseudouridine synthase 7
Pseudouridylation
Pus7
RNA modification
RNA processing
RNA Processing, Post-Transcriptional
RNA, Fungal - genetics
RNA, Ribosomal - genetics
rRNA
rRNA processing
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins - genetics
Saccharomyces cerevisiae Proteins - metabolism
snRNA
Substrate Specificity
tRNA
Virulence
Yeast
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Summary:RNA can be modified in over 100 distinct ways, and these modifications are critical for function. Pseudouridine synthases catalyse pseudouridylation, one of the most prevalent RNA modifications. Pseudouridine synthase 7 modifies a variety of substrates in Saccharomyces cerevisiae including tRNA, rRNA, snRNA, and mRNA, but the substrates for other budding yeast Pus7 homologues are not known. We used CRISPR‐mediated genome editing to disrupt Candida albicans PUS7 and find absence leads to defects in rRNA processing and a decrease in cell surface hydrophobicity. Furthermore, C. albicans Pus7 absence causes temperature sensitivity, defects in filamentation, altered sensitivity to antifungal drugs, and decreased virulence in a wax moth model. In addition, we find C. albicans Pus7 modifies tRNA residues, but does not modify a number of other S. cerevisiae Pus7 substrates. Our data suggests C. albicans Pus7 is important for fungal vigour and may play distinct biological roles than those ascribed to S. cerevisiae Pus7.
ISSN:0749-503X
1097-0061
DOI:10.1002/yea.3436