3D-printed biological cell phantom for testing 3D quantitative phase imaging systems

As the 3D quantitative phase imaging (QPI) methods mature, their further development calls for reliable tools and methods to characterize and compare their metrological parameters. We use refractive index engineering during two-photon laser photolithography to fabricate a life-scale phantom of a bio...

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Bibliographic Details
Published in:Scientific reports 2019-12, Vol.9 (1), p.18872-9, Article 18872
Main Authors: Ziemczonok, Michał, Kuś, Arkadiusz, Wasylczyk, Piotr, Kujawińska, Małgorzata
Format: Article
Language:English
Subjects:
132/124
639/624/1107/1109
639/624/1107/328/1651
Accuracy
Animals
Calibration
Geometry
Holography
Humanities and Social Sciences
Humans
Lasers
Light
Mammalian cells
Measurement techniques
multidisciplinary
Phantoms, Imaging
Photolithography
Printing, Three-Dimensional
Scanning electron microscopy
Science
Science (multidisciplinary)
Standard deviation
Tomography
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Summary:As the 3D quantitative phase imaging (QPI) methods mature, their further development calls for reliable tools and methods to characterize and compare their metrological parameters. We use refractive index engineering during two-photon laser photolithography to fabricate a life-scale phantom of a biological cell with internal structures that mimic optical and structural properties of mammalian cells. After verification with a number of reference techniques, the phantom is used to characterize the performance of a limited-angle holographic tomography microscope.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-019-55330-4