A decade of test-retest reliability of functional connectivity: A systematic review and meta-analysis

• Published in: NeuroImage (Orlando, Fla.) Fla.), 2019-12, Vol.203, p.116157-116157, Article 116157
• Main Authors: Noble, Stephanie, Scheinost, Dustin, Constable, R. Todd
• Format: Article
• Language: English
• Subjects: Brain - diagnostic imaging; Brain - physiology; Brain Mapping; Brain research; Cortex; Edge-level; fMRI; Functional magnetic resonance imaging; Humans; Image Processing, Computer-Assisted - methods; Intraclass correlation coefficient; Magnetic Resonance Imaging; Meta-analysis; Nervous system; Neural networks; Neural Pathways - diagnostic imaging; Neural Pathways - physiology; Noise; Reproducibility of Results; Reviews; Systematic review; Validity
• ISSN: 1053-8119; 1095-9572; 1095-9572
• DOI: 10.1016/j.neuroimage.2019.116157

Once considered mere noise, fMRI-based functional connectivity has become a major neuroscience tool in part due to early studies demonstrating its reliability. These fundamental studies revealed only the tip of the iceberg; over the past decade, many test-retest reliability studies have continued to add nuance to our understanding of this complex topic. A summary of these diverse and at times contradictory perspectives is needed. We aimed to summarize the existing knowledge regarding test-retest reliability of functional connectivity at the most basic unit of analysis: the individual edge level. This entailed (1) a meta-analytic estimate of reliability and (2) a review of factors influencing reliability. A search of Scopus was conducted to identify studies that estimated edge-level test-retest reliability. To facilitate comparisons across studies, eligibility was restricted to studies measuring reliability via the intraclass correlation coefficient (ICC). The meta-analysis included a random effects pooled estimate of mean edge-level ICC, with studies nested within datasets. The review included a narrative summary of factors influencing edge-level ICC. From an initial pool of 212 studies, 44 studies were identified for the qualitative review and 25 studies for quantitative meta-analysis. On average, individual edges exhibited a “poor” ICC of 0.29 (95% CI = 0.23 to 0.36). The most reliable measurements tended to involve: (1) stronger, within-network, cortical edges, (2) eyes open, awake, and active recordings, (3) more within-subject data, (4) shorter test-retest intervals, (5) no artifact correction (likely due in part to reliable artifact), and (6) full correlation-based connectivity with shrinkage. This study represents the first meta-analysis and systematic review investigating test-retest reliability of edge-level functional connectivity. Key findings suggest there is room for improvement, but care should be taken to avoid promoting reliability at the expense of validity. By pooling existing knowledge regarding this key facet of accuracy, this study supports broader efforts to improve inferences in the field. •We present the first meta-analytic review of functional connectivity reliability.•On average, individual connections exhibit a “poor” ICC of 0.29.•Study characteristics that influence reliability are summarized and discussed.•Findings suggest room for improvement and need to also consider validity.