MicroRNA-497-5p Induces Cell Cycle Arrest Of Cervical Cancer Cells In S Phase By Targeting CBX4

miR-497-5p can inhibit cervical cancer cell proliferation. However, the underlying mechanism remains to be elucidated. Bioinformatics was used to analyze the target genes of miR-497-5p. qRT-PCR and Western blot were used to analyze mRNA and protein expression, respectively. Dual-luciferase reporter...

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Bibliographic Details
Published in:OncoTargets and therapy 2019-12, Vol.12, p.10535-10545
Main Authors: Chen, Yani, Du, Juan, Wang, Yu, Shi, Haiyan, Jiang, Qiuyu, Wang, Yangfeng, Zhang, Huahua, Wei, Yameng, Xue, Wanjuan, Pu, Zhiying, Gao, Yi, Li, Dan, Feng, Yun, Yan, Jing, Zhang, Jing
Format: Article
Language:English
Subjects:
Anopheles
Cancer cells
Cancer research
Carcinoma
Cell cycle
Cervical cancer
Computational biology
Genes
Journalists
Messenger RNA
MicroRNA
Original Research
Proteins
RNA
RNA interference
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Summary:miR-497-5p can inhibit cervical cancer cell proliferation. However, the underlying mechanism remains to be elucidated. Bioinformatics was used to analyze the target genes of miR-497-5p. qRT-PCR and Western blot were used to analyze mRNA and protein expression, respectively. Dual-luciferase reporter assay was used to analyze the direct binding between miR-497-5p and 3'-untranslated region of CBX4. Cell viability was measured with MTT assay. Flow cytometry was performed to detect cell cycle distribution. Here, using bioinformatics methods we firstly found that miR-497-5p regulated cervical carcinoma proliferation by targeting polycomb chromobox4 (CBX4). Expression of miR-497-5p in cervical carcinoma tissues was negatively correlated with CBX4. A binding region of miR-497-5p in 3'-untranslated region of CBX4 was predicted. Further experiments confirmed that miR-497-5p directly targeted CBX4. Besides, RNA interference of CBX4 inhibited cervical cancer cell proliferation, arrested cells at S phase and reduced the expression of CDK2 and Cyclin A2 proteins. The use of miR-497-5p inhibitor compromised CBX4 interference RNAs induced cycle arrest of cervical cancer cells. Cells co-transfected with miR-497-5p inhibitors and CBX4 interference RNAs had a higher proliferation rate than CBX4 inference RNA-transfected cells. All together, the present study demonstrates that miR-497-5p inhibits cervical cancer cells proliferation by directly targeting CBX4.
ISSN:1178-6930
1178-6930
DOI:10.2147/OTT.S210059