IL-36γ is a pivotal inflammatory player in periodontitis-associated bone loss

• Published in: Scientific reports 2019-12, Vol.9 (1), p.19257-12, Article 19257
• Main Authors: Cloitre, Alexandra, Halgand, Boris, Sourice, Sophie, Caillon, Jocelyne, Huck, Olivier, Bugueno, Isaac Maximiliano, Batool, Fareeha, Guicheux, Jérôme, Geoffroy, Valérie, Lesclous, Philippe
• Format: Article
• Language: English
• Subjects: 13; 13/106; 13/21; 38; 38/77; 631/250/808; 692/699/3020/3029; 82; 82/51; 82/80; Alveolar Bone Loss - metabolism; Alveolar Bone Loss - microbiology; Alveolar Bone Loss - pathology; Bacteroidaceae Infections - metabolism; Bacteroidaceae Infections - pathology; Biochemistry, Molecular Biology; Bone loss; Cell Line; Chronic illnesses; Cytokines; Disease; Female; Genomics; Gum disease; Humanities and Social Sciences; Humans; Immunology; Inflammation; Inflammation - metabolism; Inflammation - microbiology; Inflammation - pathology; Innate immunity; Interleukin-1 - metabolism; Life Sciences; Male; multidisciplinary; Pathogens; Periodontitis; Periodontitis - metabolism; Periodontitis - microbiology; Periodontitis - pathology; Porphyromonas gingivalis - metabolism; Science; Science (multidisciplinary); Tumor necrosis factor-TNF
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-019-55595-9

Periodontitis is a prevalent chronic inflammatory disease due to the host response (IL-1β, IL-6, TNF-α and IL-17A) to oral bacteria such as Porphyromonas gingivalis . The newer members of the IL-1 family, IL-36s (IL-36α/IL-36β/IL-36γ/IL-36Ra/IL-38) are known to be involved in host defense against P. gingivalis in oral epithelial cells (OECs) and are considered as key inflammatory mediators in chronic diseases. The aim of this study was to investigate the potential role of IL-36s in periodontitis. We showed here that IL-36γ mRNA gingival expression is higher in periodontitis patients, whereas IL-36β and IL-36Ra mRNA expression are lower compared to healthy controls. Interestingly, the elevated IL-36γ expression in patients is positively correlated with the RANKL / OPG ratio, an index of bone resorption. In vitro, IL-36γ expression was induced through TLR2 activation in primary OECs infected with P. gingivalis but not in gingival fibroblasts, the most widespread cell type in gingival connective tissue. In OECs, recombinant IL-36γ enhanced the expression of inflammatory cytokines ( IL-1β , IL-6, TNF-α and IL-36γ ), of TLR2 and importantly, the RANKL / OPG ratio. These findings suggest that IL-36γ could be a pivotal inflammatory player in periodontitis by perpetuating gingival inflammation and its associated alveolar bone resorption and could be a relevant therapeutic target.