Correlation between PTEN and P62 gene expression in rat colorectal cancer cell

• Published in: Saudi journal of biological sciences 2019-12, Vol.26 (8), p.1986-1990
• Main Authors: Zhang, Li-ze, Qi, Wen-hai, Zhao, Gang, Liu, Lin-xun, Xue, Hui, Hu, Wen-xiu, Wang, Qian-qian, Li, Chun-sheng
• Format: Article
• Language: English
• Subjects: Autophagy; Colorectal cancer; p62; pEGFP-N1-PTEN plasmid; PTEN gene; Regulation; الأورام; الخلايا السرطانية; السرطان; القولون; المستقيم
• ISSN: 1319-562X; 2213-7106
• DOI: 10.1016/j.sjbs.2019.08.006

Autophagy is a cellular pathway that regulates the transportation and degradation of cytoplasmic macromolecules and organelles towards lysosome, which is often related to the tumorigenesis and tumor suppression. Here, we investigate the regulating effect of PTEN gene on autophagy-related protein P62 in rat colorectal cancer (CRC) cells and explore the application value of PTEN gene in clinic. Rat colorectal cancer was induced by intraperitoneal injection of 1,2-dimethyl hydrazine in male ACI rats. A total of 20 rats were randomly selected from those successfully induced with CRC as the experimental group, while 10 healthy rats as control. The rat CRC cells were isolated and cultured. After transfecting the rat CRC cells with pEGFP-N1-PTEN plasmid, RT-PCR was adopted to examine that gene expression of p62 and PTEN, while Western blotting was used to detect the protein expression of p62 and PTEN. Also, the proliferation of CRC cells was measured by MTT assay. The expression of PTEN gene in the experimental group was significantly inhibited as compared with the control group, while the expression of P62 gene was significantly increased (p