Effect of Sintering on In Vivo Biological Performance of Chemically Deproteinized Bovine Hydroxyapatite

• Published in: Materials 2019-11, Vol.12 (23), p.3946
• Main Authors: De Carvalho, Bruno, Rompen, Eric, Lecloux, Geoffrey, Schupbach, Peter, Dory, Emilie, Art, Jean-François, Lambert, France
• Format: Article
• Language: English
• Subjects: Adsorption; Animals; Biological properties; Biomedical materials; bone regeneraton; Crystal defects; Crystallites; Dentisterie & médecine buccale; Dentistry; Dentistry & oral medicine; Heat treatment; Hogs; Human health sciences; Hydroxyapatite; Investigations; Laboratories; Microscopy; osteoconduction; Regeneration (physiology); Scanning electron microscopy; Sciences de la santé humaine; Sintering; Surface area; surface microtopograraphy; Surface roughness; Surgery; Surgical implants; Transplants & implants
• ISSN: 1996-1944; 1996-1944
• DOI: 10.3390/ma12233946

The influence of the manufacturing process on physicochemical properties and biological performance of xenogenic biomaterials has been extensively studied, but its quantification on bone-to-material contact remains poorly investigated. The aim of this study was to investigate the effect of different heat treatments of an experimental chemically-deproteinized bovine hydroxyapatite in vivo in terms of new bone formation and osteoconductivity. Protein-free hydroxyapatite from bovine origin was produced under sub-critical conditions and then either sintered at 820 °C or 1200 °C. Structural and morphological properties were assessed by scanning electron microscopy (SEM), measurement of surface area and X-ray diffractometry (XRD). The materials were then implanted in standardized alveolar bone defects in minipigs and histomorphometric evaluations were performed using non-decalcified sections. Marked topographical differences were observed by SEM analysis. As the sintering temperature of the experimental material increased, the surface area significantly decreased while crystallite size increased. In vivo samples showed that the highly sintered BHA presented a significantly lower percentage of newly formed bone than the unheated one ( = 0.009). In addition, the percentage of bone-to-material contact (BMC) was significantly lowered in the highly sintered group when compared to the unsintered ( = 0.01) and 820 °C sintered ( = 0.02) groups. Non-sintered or sintered at 820 °C BHA seems to maintain a certain surface roughness allowing better bone regeneration and BMC. On the contrary, sintering of BHA at 1200 °C has an effect on its morphological and structural characteristics and significantly modify its biological performance (osteoconductivity) and crystallinity.